... HMW) - (-NmTm), (-NmTm w.t.) -NmTm, R91G (-NmTm R91G). ... NmTm w.t. ( ) -NmTm R91G ( ), 222 (). 1 /, ­ 0,1 /. : 10 - (pH 7.3), 100 mM NaCl, 1 mM MgCl2 1 mM -ME. ... 46 F-, 70 , 30 mM Hepes, 100 mM KCl, 1 mM MgCl2 1 mM -ME, pH 7,3. 1 /. o 50 42 -NmTm WT (Tdiss = 37,2 C) -NmTm R91G (Tdiss = 30,3 C) o o , % 100 80 60 40 20 0 20 25 30 35 40 o 45 50 , C . ... SkTm WT -SkTm R91G NmTm -SkTm, . ... SkTm w.t. ( ) -SkTm R91G ( ), 222 (). 1 /, ­ 0,1 /. : 10 - (pH 7.3), 100 mM NaCl, 1 mM MgCl2 1 mM -ME. ...