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Cell. ... In cells overexpressing MycN, as well as cells in which MycN was switched off, a-TOS stimulated rapid entry of Ca2? into the cytosol, compromised Ca2? buffering capacity of the mitochondria and sensitized them towards mitochondrial permeability transition and subsequent apoptotic cell death. ... 4 Mitochondrial Ca2? accumulation in NB cells with different level of MycN expression. a Accumulation of Ca2? by mitochondria in digitonin-permeabilized MycN(?) cells. ...