... Using Raman spectroscopy the changes in conformation of haemoglobin haemoporphyrin (HH) under haemoglobin (Hb) affinity to O2 modifications (acidic pH, 2,3-diphosphoglycerate (2,3-DPG)) was investigated. The typical Raman spectra of HH at pH 7.4 (dotted line) and pH 5 (solid line) is shown in figure A. We have shown that acidic pH and 2,3-DPG actions changes of HH conformation and decreases Hb affinity to oxygen. ... The HH conformation changes will discuss (fig. ... 2007 Biophysics dep., ...