... BIO-313, Biosystems Department Danish Polymer Centre, Risoe National Laboratory, Technical University of Denmark, DK-4000 Roskilde, Denmark . ... 2 Department of Physics, Stanford University, Stanford, CA 94305, USA . ... The limited number of photons available from a single fluorescent molecule makes optimal data analysis is as important to experimental resolution as improved experimental conditions, such as photo bleaching rates of fluorescent probes. ... 2007 Biophysics dep., ...