Nucleotide BLAST . ... These searches translate either query sequences or databases from nucleotides to proteins so that protein - nucleotide sequences can be performed. ... Takes a protein query sequence and compares it against an NCBI nucleotide database which has been translated in all six reading frames. ... Converts a nucleotide query sequence into protein sequences in all 6 reading frames and then compares this to an NCBI nucleotide database which has been translated in all six reading frames. ...