... Why does my search timeout on the BLAST servers? Why do I get the error message "ERROR: BLASTSetUpSearch: Unable to calculate Karlin-Altschul params , check query sequence"? ... What is the Expect (E) value ? ... Certain combinations of BLAST searches with large sequences against large databases can cause the BLAST servers to timeout. ... You can use the BLAST 2.2.22+ Sequences service to compare two nucleotide or two protein sequences against each other using the BLAST 2.2.22+ algorithm. ...