... The primary monoclonal antibodies specific to the N-terminal domain of human ER protein (clone 14C8, Abcam) and the secondary FITC-conjugated antibodies (F2772, Sigma) were used in the investigation. ... Human breast adenocarcinoma cell line MCF-7 and the range of the primary antibody concentrations 0.06, 1.2 and 2.5 µg/ml is recommended for the regular antibody activity control in clinical using. Moscow University Chemistry Bulletin . 2011, Vol. ... 4, P. 305 . ...