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I .., .. () 1 (PSI) . Fd- PSI. 700 Fd .
MODELING OF CYCLIC ELECTRON TRANSPORT AROUND PHOTOSYSTEM 1

Kovalenko I.B., Riznichenko G.Yu. (Moscow) Cycle electron transport around photosystem I (PSI) in chloroplasts of higher plants and algae is considered. A series of kinetic models of ferredoxin-dependent cyclic electron transport around PSI is suggested. The investigation of the kinetic models of cycle electron transport allowed us to describe the set of experimental data for dependence of kinetic parameters of P700 reduction at different concentrations of exogenic ferredoxin in the context of the scheme of interaction of intermembrane multiprotein complexes and mobile carriers. 1 (PSI) , 232


.. . -- -10, 2002, .232-233

NADPH, - PSI . , . , PSI, . 1. PSI (Pc) (Fd) , , ( ). Fd , PQ , , , Fd-PQ-- (FQR) [1-4]. PQ Pc ( ), b6/f Q- .

. 1. . : PSI, FQR, FNR b6/f, Pc, Fd Q. , .

. .. . .. 233


2. ,

I, 700 ­ PSI [5], 0,1-10 . I, .. 700, (. 2). .

. 2. 700. ­ P700+ A(t) = A1exp(­k1t)+A2exp(­k2t), A1, A2 ­ , , k1, k2 , . .

I : ­ -

. 1 700+ Fd: , . , 200 , 2-5 . Fd 700+, (,
234


.. . -- -10, 2002, .232-235

) . Fd , 700+, , . Fd. [6] , 700 «» 700 .
1. 700+ Fd. A1 A2 ­ , , k1 k2 ­ , 1 2 ­ . A 1, k1, ­1 k2, ­1 2, A 2, A1/ 1, .. .. (A1+ Fd, A2) 0 0.240 6.85 0.146 0.361 0.181 5.51 0.33 5 0.166 4.93 0.203 0.276 0.218 4.57 0.35 10 0.458 4.49 0.222 0.192 0.514 1.94 0.74 15 0.593 4.28 0.233 0.163 0.428 2.33 0.80 20 0.656 4.23 0.236 0.159 0.464 2.15 0.86 -

, Fd PSI , (. 1) 0.1 ­ 10 . 700+ . - , - PSI, - . PSI 235


2. ,

[7-9]. . , (0.1 ). , PSI , 700 . , . - , (Pc, PQ, Fd), . b6/f FQR .

. 3. PSI. 700 ­ PSI, ­ , Fd ­ , Pc ­ , PQ ­ . , kin, kout, k1, ..., k4 ­ . .

Model Vision Studium [10]. P700+ . ,
236


.. . -- -10, 2002, .232-237

. «» k0 : k0 = I ,

I ­ , - PSI. , 500 /2 ( ) k0 = 250 -1. Pc P700: k1 = 4103; Pc Q: k4 = 50 [11, 12]; PSI Fd: k2 = 5105 [4]. . 2 6. 10% Fd . 700+ . 4. 700+, , . , . , 700+, . . , ­ . , ­ . Fd PSI, PQ, PQ . . 5, FQR. PSI . 6. , PSI , 237


2. ,

700 , Fd.

. 4. - 700+. Fd0 ­ Fd PSI.

b6/f (. 6) , R bh, Qn. Q-. Fd Fd . , FdPQ- - FQR (. 6), 6 .

238


.. . -- -10, 2002, .232-239

. 5. PSI. PSI, FQR b6/f . 700 ­ PSI, ­ , R ­ , b ­ bh, Fd ­ , Pc ­ , Q ­ , Q­ ­ ­ , Qn ­ n- ( ). , kin, kout, k1, ..., k14 ­ . .







. 6. , . , - . , kin, kout, k1, ..., k14 ­ . ) PSI. 700 ­ PSI, ­ . ) b6/f . R ­ , b ­ b, Qn ­ n ( ). ) FQR.

, (. 5, 6), 26 . 239


2. ,

, Fd, PQ . . . «» k0 = 250 (. ). : k4 = 50, k5 = 457, k6 = 10000, k7 = 4000 [12]. Pc P700: k1 = 4000, Fd PSI: k11 = 5105 [11, 12]. . 1, 700 , Fd, Pc, PQ, FQR cyt b6/f, « » . . 1 700 Fd 0,1-10 . (.1). , 700 : ( ) () . Fd (. 5). , Fd , Fd, PSII ­ . 240


.. . -- -10, 2002, .232-241

P700 , . , , PQ , . ( ­ ) [2, 3, 6]. , «» Fd. «» 700. 700+ , «» 700. , . PSI [4, 13]. 700 , . [6] Scheller , P700 P700+ « », .. . , , PSI. [14] Hope P700+. , «». , , . .
241


2. , . - . .., .., .. . . . .. . ( 03-04-49048, 01-07-90131).

. 1. Allen, J.F., Cyclic, pseudocyclic and noncyclic photophosphorylation: new links in the chain. TRENDS in Plant Science, 2003. 8(1): p. 15-19. 2. Bendall, D.S. and R.S. Manasse, Cyclic photophosphorylation and electron transport. Biochimica et Biophysica Acta, 1995. 1229: p. 23-38. 3. Cleland, R.E. and D.S. Bendall, Photosystem I cyclic electron transport: Measurement of ferredoxin-plastoquinone reductase activity. Photosynth. Res., 1992. 34: p.409-418. 4. Malkin, R. and K. Niyogi, Photosynthesis, in Biochemistry & Molecular Biology of Plants, B. Buchanan, W. Gruissem, and R. Jones, Editors. 2000, American Society of Plant Physiologists. 5. .., .., .., .., .. --. , 2001. 379(5): p. 1-4. 6. Scheller, H.V., In vitro cyclic electron transport in barley thylakoids follows two independent pathways. Plant Physiology, 1996. 110: p. 187-194. 7. , .. . , 1991. 31. 8. , .. . 2 . .2. 2000. .: "". 9. , .., .. , .. , . 1987, .: - . 10. .., .., .. . : , 2002.
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11. Hope, A.B., Electron transfers amongst cytochrome f, plastocyanin and photosystem I: kinetics and mechanisms. Biochimica et Biophysica Acta, 2000. 1456: p. 5-26. 12. Hope, A.B., J. Liggins, and D.B. Matthews, The kinetics of reactions in and near the cytochrome b/f complex of chloroplasts. Cytochrome b-563 reduction. Aust. J. Plant Physiol., 1989. 16: p. 353-364. 13. , . . 1. .: "", 1987. 14. Hope A.B., Huilgol, R.R., Panizza M.,Thompson, M., Matthews, D.B. The flash-induced turnover of cytochrome b-563, cytochrome f and plastocyanin in chloroplasts. Models and estimation of kinetic parameters. Biochimica et Biophysica Acta, 1992. 1100: p. 15-26.

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