Документ взят из кэша поисковой машины. Адрес оригинального документа : http://www.mce.biophys.msu.ru/archive/doc60858/doc.pdf
Дата изменения: Tue Mar 9 12:28:51 2010
Дата индексирования: Mon Oct 1 22:47:17 2012
Кодировка:

a . ., . ., . ., ., .. . ., .,

,

. B., . .,

.

-

.

Fv/Fm , . F / F m' . , 2, . ,

-

Fv/Fm

-

3, .

,

. . . , ( , . , , ), , . , , , . .. , , (.. , , ) , -

180

. ., . ., . B., ... -- 2009, . 2, . 180197 Volgusheva A. A., Yakovleva O. V., Konyukhov I. V., ... -- MCE 2009, v. 2, pp. 180197

. , . . , , , , .

( , 2000; Adams and Demming-Adams, 2004; Cavender-Bares and Bazzaz, 2004; ., 2005). ,, , . , -- J I, (Fo) (Fm) (Strasser et al., 2004). JIP(Strasser and Strasser, 1995) , . , (Srivastava et al., 1997), (Bussotti et al., 2005), ( ., 2008). , . , . ( , )
181

(KrЭger et al., 1997), (Ali et al., 2006) , , -

6. . Part 6. Analysis of Complex Biological Systems. Models and Experiment

, ( ., 1999). ( ( » ( ). , . , , , . . , 20 200 . . , ), , , « , , ), -

tion) («Walz, Effelrich»,

PAM-2000 (Pulse Amplitude Modula). , . ,

10 . 30 . : Fo --

0.1 3 = 655 ); Fm -- 0.8 < 710 , 1100
-2 -1

600

( Q, (20

, H-3000 Stanley,
-2 -1

Q ).

,

182

. ., . ., . B., ... -- 2009, . 2, . 180197 Volgusheva A. A., Yakovleva O. V., Konyukhov I. V., ... -- MCE 2009, v. 2, pp. 180197

( , . 470 5 Fm 2 Fm' ( = 470 ) 3 ), -79 Fm' 70 (Luxeon). (

) , 0.8 -18. (6000
-2 -1

,

) Ft

-2 -1

-22.
-2 -1

400

. 2

Fv/Fm = (FmFo)/Fm, (Shreiber et al., 1995).

F/Fm' = (Fm'Ft)/Fm' (NPQ) NPQ = Fm/Fm'1 (Gilmore and BjЖrkman, 1994). (Plant Efficiency Analyzer) (Hansatech, ( = 650 ) , .

King's Lynn, Norfolk, UK). 3000
-2 -1

.

JIP, (Strasser and Strasser, 1995). : (Fo J(Fv) ); ) 30 ); 300 µ (F300µ ), (F30 , I). Fv = FmFo. , JIP, . , 2

(Fm (F2 ,

JIP-

(

. 1).

183

6. . Part 6. Analysis of Complex Biological Systems. Models and Experiment

1. VJ = (F VI = (F
2

JIP-

. OJ , JI -

Fo)/Fv Fo)/Fv , cQ
300µ -

30

,

ETO/TRO = 1 VJ RC/ABS = [(F2 Fo)/4(F Fo)] (Fv/Fm)

, / ,

P

TR

= Fv/Fo ,

, , -

P

ET

= (Fm F

2

)/(F2 Fo)

PI = (RC/ABS)(PTR)(PET)

.

-

.

., 1999; , . , , , . . 1. , . ,

2 (Fv/Fm) ( ., 2005; Lazar et al., 2006). Fv/Fm

-

, 200 Fv/Fm Fv/Fm -

-

184

. ., . ., . B., ... -- 2009, . 2, . 180197 Volgusheva A. A., Yakovleva O. V., Konyukhov I. V., ... -- MCE 2009, v. 2, pp. 180197

0.80±0.01,

, 0.79±0.02.

0.81±0.01 , , ,

-

-

,

. Fv/Fm. , , ( Fv/Fm , , Fv/Fm , . 1. ). , -

-

., 1999). . 1. -

.

, , , , Fv/Fm (

. 1. Fv/Fm , ( ), () (). . 200

-

,

185

6. . Part 6. Analysis of Complex Biological Systems. Models and Experiment

Fv/Fm -- ( . 1. ). , . , , . , . , 2 (Fv/Fm) ( F/Fm'), . , (Kuznetsova et al., 2008). 400 ( . , , , , (400
-2 -1 -2 -1

, Fv/Fm 0.74 -

-

, 3

, ). .2 , Fv/Fm -

. ,3 .) . F/Fm'

, 30%

Fv/Fm, . , . (NPQ) . , NPQ
186

, F/Fm' 40%, -

NPQ QA. . 2. ,

, ..

. ., . ., . B., ... -- 2009, . 2, . 180197 Volgusheva A. A., Yakovleva O. V., Konyukhov I. V., ... -- MCE 2009, v. 2, pp. 180197

NPQ 56%, . NPQ 2

-

.

. 2.

Fv/Fm, F/Fm'

NPQ .

-

. , .

), 2 (Lazar, 2006). QA, , QB Q -Q , Q -Q 2-, PQH2 , .. .
187

OJ JIP QA-, ,

(O-J-I-P-

O P

6. . Part 6. Analysis of Complex Biological Systems. Models and Experiment

Fo Strasser, 2004). .3

Fm (Heredia, De Las Rivas, 2003;

( ). , , 2005). Fo) . , ( .3 (VJ), , .. , OJ JI, (Schreiber et al., 1989). , 1 , -O22

,

( ), 3

() (OJ, JI, IP) 1 (Schansker et al., ( , -

, ( Fm)

Fv), 2 , QA, QQB
A

.

OJ

. , . ( ), 2 (Baena-

JIP

(

), (Kuntz, 2004). /

, , (Rizhsky et al., 2002), Gonzalez et al., 2003).

188

. ., . ., . B., ... -- 2009, . 2, . 180197 Volgusheva A. A., Yakovleva O. V., Konyukhov I. V., ... -- MCE 2009, v. 2, pp. 180197

. 3. ,

--

,

--

, ,

--
OJ (Wt)

.

. (VJ), . . Wt = Vt/VJ = (FtFo)/(FJFo).

JIPJIPJIP,

. ,

-

, (Strasser et al., 2000; 2004). , PI, 2, , (ABS), , (TR) (ET) (Strasser et al., 2000; 2004). , PI , Fv/Fm (Hermans et al., 2003; Thach et al., 2007). -

, al., 2004; Strauss et al., 2006; Thach et al., 2007). JIP, , , , ( ) 40%.
189

.4

: (De Ronde et -

. RC/ABS

-

6. . Part 6. Analysis of Complex Biological Systems. Models and Experiment

(Adams and Demming-Adams, 2004). ( ET) (40% 60%, ), (40% 50%). I , . , cQ
-

(

TR)

-

(

) -

2

(VJ), (ETo/Tro) (VI). PI (Camellia sinensis) (Lin et al., 2009). ,

2

. , . , , . 2 (QA ( , ( ( QB) , 2003). ) . 4). , . , ( PI, 2 (VJ), Q
ET)

,

,

, ,

,

, (VI)

, (ETo/Tro).

190

. ., . ., . B., ... -- 2009, . 2, . 180197 Volgusheva A. A., Yakovleva O. V., Konyukhov I. V., ... -- MCE 2009, v. 2, pp. 180197

. 4. ( . )

JIP( )

, ( ) 100/ .

:

. Fv/Fm, , , 200 . . F/Fm' , 2 . , . , , ( ., 1999).
191

, ,

-

, .

Fv/Fm

,

,

, ,

NP Q -

6. . Part 6. Analysis of Complex Biological Systems. Models and Experiment

. 5. ( ), () , , . () .

--

-

2

, -

--

,

,

-- --
.

, ,

--

--

192

. ., . ., . B., ... -- 2009, . 2, . 180197 Volgusheva A. A., Yakovleva O. V., Konyukhov I. V., ... -- MCE 2009, v. 2, pp. 180197

( ),

() , ,

() . 5.

,

, ,

. JIP2

, . ,

2, . . , , - 22 2

, , 2. -

(Baena-Gonzalez et al., 2003), 2. 2( , , 2006). . . ( -

, ,

5. ) ( ) .
2

. -

, ,

, .

,

-

-0804-90-205-853.2008.4 02.512.11.2213

_,

193

6. . Part 6. Analysis of Complex Biological Systems. Models and Experiment

. .,

.. , . -- 2006. -- . 53, . ., . ., . ., 5. -- . 645657. . ., . ., . ., I // . ., . .,

-

. ., ..

// . -- 1999. -- . 44, 6. -- . 10371047. . ., . ., . ., . ., .. hlamydomonas reinhardtii, // . .,

. //

-- 2008. -- . 53, .. . ., . .,

5. -- . 787796. . -- 2000. -- . 6, // 4. -- C. 713.

.. . -- 2004. -- . 49, . .,

2. -- . 239253. . .,

. .,

..

-

, // .-- 2005. -- . 50, 6. -- . 11121119. Adams W.W., Demming-Adams B. Chlorophyll fluorescence as a tool to monitor plant response to the environment // In: Papageogiou G, Govindjee (eds) Chlorophyll a fluorescence: a signature of photosynthesis / Advances in photosynthesis and respiration / Springer, Dordrecht. -- 2004. -- Vol. 19. -- P. 583604. Ali N. A., Dewez D., Didur O., Popovic R. Inhibition of photosystem II photochemistry by Cr is caused by the alteration of both D1 protein and oxygen evolving complex // Photosynthesis Research. -- 2006. -- August. -- Vol. 89, no. 2. -- P. 8187. Baena-Gonzalez E, Allahverdiyeva Y, Svab Z, Maliga P, Josse E. M, Kuntz M, Maenpaa P, Aro E. M. Deletion of the tobacco plastid psbA gene triggers an upregulation of the thylakoid-associated NAD(P)H dehydrogenase complex and the plastid terminal oxidase (PTOX) // Plant Journal. -- 2003. -- September. -- Vol. 35, no. 6. -- P. 704716.

194

. ., . ., . B., ... -- 2009, . 2, . 180197 Volgusheva A. A., Yakovleva O. V., Konyukhov I. V., ... -- MCE 2009, v. 2, pp. 180197

Bussotti F., Agati G., Desotgiu R., Matteini P., Tani C. Ozone foliar symptoms in woody plant species assessed with ultrastructural and fluorescence analysis // New Phytologist. -- 2005. -- January. -- Vol. 166. -- P. 941955. Cavender-Bares J., Bazzaz F.A. From leaves to ecosystems: using chlorophyll fluorescence to access photosynthesis and plant function in ecological studies // In: Papageogiou G, Govindjee (eds) Chlorophyll a fluorescence: a signature of photosynthesis / Advances in photosynthesis and respiration / Springer, Dordrecht. -- 2004. -- Vol. 19. -- P. 737755. De Ronde J.A., Cress W.A., KrЭger G.H.J., Strasser R.J., Van Staden J. Photosynthetic response of transgenic soybean plants, containing an Arabidopsis P5CR gene, during heat and drought stress // Journal of Plant Physiology. -- 2004. -- November. -- Vol. 161, no. 6. -- P. 12111224. Gilmore A.M., BjЖrkman O. Adenine nucleotides and the xanthophyll cycle in leaves. II. Comparison of the effects of CO2- and temperature-limited photosynthesis on Photosystem II fluorescence quenching, the adenylate energy charge and violaxanthin deepoxidation in cotton // Planta. -- 1994. -- Vol. 192, no. 4. -- P. 537544. Heredia P., De Las Rivas J. Fluorescence induction of photosystem II membranes shows the steps till reduction and protonation of the quinone pool // Journal of Plant Physiology. -- 2003. -- December. -- Vol. 160, no. 12. -- P. 14991506. Hermans C., Smeyers M., Rodrigues R.M., Eyletter M, Strasser R., Dehaye J.P. Quality assessment of urban's trees: a comparative study of physiological characterization, airborne imaging and on site fluorescence monitoring by the OJIP test // Journal of Plant Physiology. -- 2003. -- January. -- Vol. 160, no. 1. -- P. 8190. KrЭger G.H.J., Tsimilli-Michael M., Strasser R.J. Light stress provokes plastic and elastic modifications in structure and function of Photosystem II in camellia leaves // Physiology Plantarum. -- 1997. -- Vol. 101, no. 2. -- P. 265277. Kuntz M. Plastid Terminal Oxidase and Its Biological Significance // Planta. -- 2004. -- Vol. 218, no. 6. -- P. 896899. Kuznetsova N.I., Azizbekyan R.R., Konyukhov I.V., Pogosyan S.I., Rubin A.B. Inhibition of photosynthesis in cyanobacteria and plankton algae by the bacterium Brevibacillus laterosporus metabolites // Doklady Biochemistry and Biophysics. -- 2008. -- . 421, no. 2. -- P. 262266. Lazar D., Susila P., Naus J. Early detection of plant stress from changes in distributions of chlorophyll a fluorescence parameters measured with fluorescence imaging // Journal of Fluorescence. -- 2006. -- Vol. 16, no. 2. -- P. 173176. Lin Z-H., Chen L-S., Chen R-B., Zhang F-Z., Jiang H-X and Tang N. CO2 assimilation, ribulose-1,5-bisphosphate carboxylase/oxygenase, carbohydrates and
195

6. . Part 6. Analysis of Complex Biological Systems. Models and Experiment

photosynthetic electron transport probed by the JIP-test, of tea leaves in response to phosphorus supply // BMC Plant Biology. -- 2009. -- April. -- Vol. 21. -- P. 943. Rizhsky L, Hallak-Herr E, Van Breusegem F, Rachmilevitch S, Barr JE, Rodermel S, Inze D, Mittler R. Double antisense plants lacking ascorbate peroxidase and catalase are less sensitive to oxidative stress than single antisense plants lacking ascorbate peroxidase or catalase // Plant Journal. -- 2002. -- July. -- Vol. 32, no. 3. -- P. 329342. Schansker G., TСth S.Z., Strasser R.J. Methylviologen and dibromothymoquinone treatments of pea leaves reveal the role of photosystem I in the Chl a fluorescence rise OJIP // Biochimica et Biophysica Acta. -- 2005. -- Vol. 1706, no. 3. -- P. 250261. Schreiber U., Neubauer C., Klughammer C. Devices and Methods for RoomTemperature Fluorescence Analysis // Philosophical Transactions of the Royal Society of London / Series B, Biological Sciences. -- 1989. -- Vol. 323. -- P. 241251. Srivastava A., Guisse B., Greppin H., Strasser R. J. Regulation of antenna structure and electron transport in PS II of Pisum sativum under elevated temperature probed by the fast polyphasic chlorophyll a fluorescence transient OKJIP // Biochimica et Biophysica Acta. -- 1997. -- Vol. 1320, no. 1. -- P. 95106. Strasser B.J., Strasser R.J. Measuring fast fluorescence transients to address environmental questions: The JIP-test // In: Mathis P (ed) Photosynthesis: From Light to Biosphere / Kluwer Academic Publishers / Dordrecht. -- 1995. -- August. -- P. 977980. Strasser R.J., Srivastava A., Tsimilli-Michael M. The fluorescence transient as a tool to characterize and screen photosynthetic sample // In: Yunus M, Pathre U, Mohanty P (eds) Chlorophyll a fluorescence: a signature of photosynthesis / Advances in photosynthesis and respiration / Dordrecht. -- 2000. -- August. -- Vol. 19 -- P. 321362. Strasser R.J., Tsimilli-Michael M., Srivastava A. Analysis of chlorophyll a fluorescence transient // In: Papageorgiou GC and Govindjee (eds.) Chlorophyll fluorescence: a signature of photosynthesis / Kluwer Academic Publishers, Dordrecht/Boston/London. -- 2004. -- P. 321362. Strauss A.J., KrЭger G. H.J., Strasser R.J., Van Heerden P.D.R. Ranking of dark chilling tolerance in soybean genotypes probed by the chlorophyll a fluorescence transient O-J-I-P // Environmental and experimental botany . -- 2006. -- Vol. 56, no. 2. -- P. 147157.

196

. ., . ., . B., ... -- 2009, . 2, . 180197 Volgusheva A. A., Yakovleva O. V., Konyukhov I. V., ... -- MCE 2009, v. 2, pp. 180197

Thach L. B., Shapcott A., Schmidt S., Critchley C. The OJIP fast fluorescence rise characterizes Craptophyllum species and their stress responses // Photosynthesis Research. -- 2007. -- May. -- Vol. 94, no. 2. -- P. 423436.

ANALYSIS OF THE CHLOROPHYLL FLUORESCENCE PARAMETERS IN THE BARK CHLOROPLASTS OF POPLAR FROM DIFFERENT AREAS OF ULAN BATOR Volgusheva A. A., Yakovleva O. V., Konyukhov I. V., Riznichenko G. Yu., Krendeleva T. E., Gun -Aajav T., Bordanova O. S., Battulga Ch., Rubin A. B.

Fluorescence methods were used for monitoring of poplar from different areas of Ulan Bator. The Fv/Fm ratio was similar in control and annual shoots of trees from the city which indicated a relatively favourable ecological situation. However, histograms of the values Fv/Fm distribution and decreased F/Fm' ratio indicate deterioration of the physiological state of these trees. Analysis of the chlorophyll fluorescence transients showed changes of redox state of plastoquinone pool, electron transport rate in photosystem II, decrease of the size of the Chl. antenna and the number of active photosystem II reaction centers. Derangements of photosynthetic process appeared only in triennial shoots. This fact suggests about applicability of fluorescence probing of the three-years old tree shoots for evaluation physiological state of the whole plant as well as for monitoring ecological state of the phytocenosis.

197