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E. M. Basova, V. M. Ivanov, O. C. Apendeeva
Potential
of Fluorat-02 Panorama spectrofluorImeter for the analysis of fluorescent dyes
mixtures
Abstract
The fluorescent dyes fluoresceine disodium, eosin Y, rhodamine B, rhodamine 6G and amino-G acid
were chosen for the investigation. Their absorption, excitation and
fluorescence spectra in aqueous media at different pH values were recorded on a
Fluorat-02-Panorama spectrofluorimeter and the maxima of excitation and
emission for individual compound were established. The excitation and emission
maxima of fluorescein disodium, eosin Y. rhodamine B, rhodamine 6G and AGA were
490/514, 516/544, 529/555, 545/576 and 300/448 nm respectively under optimal pH
9,18 (borate buffer). The optimal conditions of simultaneous determination up
to 5 fluorescent dyes were established. The fluorescent spectra of dyes studied
overlapped. The determination of 5 dyes may be performed from fluorescence
spectra at excitation wavelength 300 nm with heart cutting of wavelength range
480-580 nm. The determination of 4 dyes (except AGA) may be performed from
fluorescence spectra at excitation wavelength 490 nm with heart cutting of
wavelength range 500-500 nm or from synchronous fluorescence spectra with 25 nm
interval between excitation and emission wavelength in the range 300-680 nm.
The dye concentrations were calculated by Panorama pro. Version 2.1.0 software.
The method was applied to analyze different model mixtures containing 2-5 dyes
with satisfactory results.
Key words: fluorescent
dyes, fluoresceine disodium, eosin Y, rhodamine B, rhodamine 6G , amino-G acid, synchronous fluorescence
spectra, simultaneous determination.
Copyright (C) Chemistry Dept., Moscow State University, 2002
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