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M. M. Ulyashova, Yu. Yu. Ryabova, M. Yu. Rubtsova, A. M. Egorov
DNA-microarrays
on porous membrane supports with colorimetric detection
Abstract
The technology of DNA-microarrays on porous membrane supports with
colorimetric detection based on horseradish peroxidase was developed. The
comparison of the methods of oligonucleotide immobilization on chemically
different membranes was carried out, the conditions of colorimetric detection
of biotin in DNA duplexes on microarray surface were optimized. The method
developed was applied for determining type of genes encoding extended-spectrum b-lactamases.
Copyright (C) Chemistry Dept., Moscow State University, 2002
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