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I. S. Alpeeva, M. M. Anokhina, V. N. Tashlitsky, A. M. Kopylov
Determination of the part of transcription repressor protein TetR(D)
active to tetra cycline-binding by competitive binding method
Abstract
The competitive binding isotherms of tetracycline and radioactive
7-[3H]-tetracycline with transcription represser protein TetR(D) were obtained.
Mathematical model of the competitive binding which enables to calculate the
part of TetR(D) active to tetracycline-binding were proposed. The application
of this correction for calculation of apparent association constants allows the
data obtained for different protein preparation to be unified. This method can
be applied for calculation of apparent association constants for various
protein complexes.
Copyright (C) Chemistry Dept., Moscow State University, 2002
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