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Tishkov V. I.
The Coenzyme Regeneration for Biosynthesis of Chiral Compounds Using
Dehydrogenases
Abstract
Principles of synthesis of chiral compounds using dehydrogenases as the
main biocatalyst and formate dehydrogenase as biocatalyst for coenzyme
regeneration have been discussed. Process of preparation of new generation of
biocatalysts for NAD(P)H regeneration using formate dehydrogenase (FDH, EC
1.2.1.2) from Pseudomonas sp.101 have been described. New recombinant
E.coli strains producing different forms of FDH have been constructed and
efficient and cheap procedure for enzyme purification have been developed.
Protein engineering approaches have been used to prepare different forms of FDH
with improved kinetic properties, increased thermal and chemical stability and
changed coenzyme specificity.
Copyright (C) Chemistry Dept., Moscow State University, 2002
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