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Balabushevich N. G., Sukhorukov G. B., Larionova N. I.
Encapsulation of Proteins into Polyelectrolytic Microcapsules Composed
of Dextran Sulfate, Protamine and Melamine Formaldehydes
Abstract
An effect of three groups of monoclonal antibodies specific to different
regions of HRP on it’s catalytic activity in the course of oxidation of ABTS
and stability during p-iodophenol (PIP) oxidation and light emission in
the ECL reaction was studied. It was found that the formation of immune
complexes of HRP and mc Ab resulted in higher catalytic activity and stability
of enzyme. The ability of mc Ab to prevent the enzyme from inactivation
depended on the antibody type. We revealed a positive effect of monAb 7G on
both maximal light intensity and overall light output.
Copyright (C) Chemistry Dept., Moscow State University, 2002
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