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T. D. Khokhlova
Influence
of surface chemistry and pore diameter of modified silicas on exclusion effects
in ovalbumin adsorption.
Abstract
Ovalbumin
was adsorbed on aerosil and series of silica gels with pore diameter 5, 14, 36
and 50 nm modified with thrimethylchlorosilane (TMCS) and
aminopropylthriethoxysilane (APTES). Influence of the silicas surface chemistry
and electrolyte (NaCl) concentration on exclusion effect in adsorption from 2%
isopropanol in 0,02 M phosphate buffer at pH 5,9 was studied. The greatest
difference in the adsorption on modified silica gels with the smallest pore
diameter 5 nm was observed. At various NaCl concentration the ovalbumin adsorption on the
anion-exchange (modified with APTES) silica gel was much more than on the
hydrophobic (modified with TMCS) one. Sources of that difference are discussed.
Copyright (C) Chemistry Dept., Moscow State University, 2002
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