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Gurevich K. G.
Mathematical modeling
application for receptor binding parameter estimation
Abstract
Mathematical modeling
was used for receptor binding parameter estimation in systems, in which
analysis with traditional methods (Scatchard, Hill, Bierrum anaysis and so on)
is much difficult or impossible: cooperative binding, presence of several binding sites, nonequilibrium binding. Using
literature it was analyzed 23 receptor systems with different chemical nature
of endogenous and exogenous ligands. It was shown that conjugate gradients method
allow to find binding parameters in all cases, in which analysis with
traditional methods is impossible or much difficult. Parameters which were
received using mathematical modeling allow to approximate experimental data
then such parameters which were received using traditional methods. It may be
suggested that mathematical modeling may be also useful for analysis of such
systems as ‘enzyme-substratum’, ‘blood protein-hormone’, ‘antigen-antibody’.
Copyright (C) Chemistry Dept., Moscow State University, 2002
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