The newest and most up-to-date collection, PCR Protocols offers detailed laboratory procedures for the use of polymerase chain reactions in a wide range of applications. Although specific examples and experimental systems are described, the book's focus is on general applications that can be modified to suit a broad spectrum of research systems, thus making its scope appealing to nearly all researchers. PCR Protocols features clear, easy-to-follow descriptions of procedures and a Notes section in each chapter to provide tips, alternative suggestions, and other enhancements of the protocols. Key Topics and Techniques: basic startup procedures for PCR  PCR in detecting DNA and RNA  PCR in DNA synthesis and mutagenesis  PCR cloning of DNA  selection of primers  radioactive and nonradioactive labeling  quantitation of PCR products by HPLC  chromosome assignment  PCR mapping of HLA genes  sequencing of PCR products  genomic footprinting  quantitation of tumor gene expression  screening of phage libraries  SSP-PCR and genome walking  subcloning of PCR products.