Документ взят из кэша поисковой машины. Адрес оригинального документа : http://kodomo.cmm.msu.ru/~student08fbb/blast.fasta Дата изменения: Sun Nov 1 18:10:48 2009 Дата индексирования: Tue Oct 2 03:31:36 2012 Кодировка:

TBLASTN 2.2.21 [Jun-14-2009]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment: Altschul, Stephen F.,
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= PTHP_ECOLI phosphohistidinoprotein-hexose phosphotransferase
component of PTS system (Hpr)
(85 letters)

Database: st
220 sequences; 4,870,572 total letters

Searching..................................................done



Score E
Sequences producing significant alignments: (bits) Value

AE008809 AE006468 Salmonella typhimurium LT2, section 113 of 220... 169 3e-44
AE008798 AE006468 Salmonella typhimurium LT2, section 102 of 220... 55 1e-09
AE008850 AE006468 Salmonella typhimurium LT2, section 154 of 220... 52 1e-08
AE008876 AE006468 Salmonella typhimurium LT2, section 180 of 220... 47 3e-07
AE008853 AE006468 Salmonella typhimurium LT2, section 157 of 220... 41 3e-05
AE008892 AE006468 Salmonella typhimurium LT2, section 196 of 220... 36 7e-04
AE008703 AE006468 Salmonella typhimurium LT2, section 11 of 220 ... 26 0.78
AE008753 AE006468 Salmonella typhimurium LT2, section 57 of 220 ... 23 5.4
AE008827 AE006468 Salmonella typhimurium LT2, section 131 of 220... 23 5.8

>AE008809 AE006468 Salmonella typhimurium LT2, section 113 of 220 of the
complete genome.
Length = 22803

Score = 169 bits (429), Expect = 3e-44, Method: Compositional matrix adjust.
Identities = 85/85 (100%), Positives = 85/85 (100%)
Frame = +3

Query: 1 MFQQEVTITAPNGLHTRPAAQFVKEAKGFTSEITVTSNGKSASAKSLFKLQTLGLTQGTV 60
MFQQEVTITAPNGLHTRPAAQFVKEAKGFTSEITVTSNGKSASAKSLFKLQTLGLTQGTV
Sbjct: 15774 MFQQEVTITAPNGLHTRPAAQFVKEAKGFTSEITVTSNGKSASAKSLFKLQTLGLTQGTV 15953

Query: 61 VTISAEGEDEQKAVEHLVKLMAELE 85
VTISAEGEDEQKAVEHLVKLMAELE
Sbjct: 15954 VTISAEGEDEQKAVEHLVKLMAELE 16028


>AE008798 AE006468 Salmonella typhimurium LT2, section 102 of 220 of the
complete genome.
Length = 20178

Score = 55.1 bits (131), Expect = 1e-09, Method: Compositional matrix adjust.
Identities = 29/78 (37%), Positives = 45/78 (57%), Gaps = 3/78 (3%)
Frame = -2

Query: 1 MFQQEVTITAPNGLHTRPAAQFVKEAKGFTSEITVTS---NGKSASAKSLFKLQTLGLTQ 57
+ E + +GLH RP V K F SEITVT+ GK A+ +SL K+ LG+ +
Sbjct: 15254 VLSAEFVVRNEHGLHARPGTMLVNTIKQFNSEITVTNLDGTGKPANGRSLMKVVALGVKK 15075

Query: 58 GTVVTISAEGEDEQKAVE 75
G + +A+GED ++A++
Sbjct: 15074 GHRLRFTAQGEDAEQALK 15021


>AE008850 AE006468 Salmonella typhimurium LT2, section 154 of 220 of
the complete genome.
Length = 21944

Score = 51.6 bits (122), Expect = 1e-08, Method: Compositional matrix adjust.
Identities = 33/84 (39%), Positives = 48/84 (57%), Gaps = 4/84 (4%)
Frame = +3

Query: 3 QQEVTITAPNGLHTRPAAQFVKEAKGFTSEITVTSNGKSAS----AKSLFKLQTLGLTQG 58
Q I P+GLH RP+A VK K F S ITV N +AS K+L ++ +LG +G
Sbjct: 5961 QATFVIHNPHGLHARPSAVLVKFIKQFQSHITV-ENLDNASGPVDGKNLMRVVSLGAKKG 6137

Query: 59 TVVTISAEGEDEQKAVEHLVKLMA 82
+ A+GED Q+A+ + +L+A
Sbjct: 6138 HRLLFRAQGEDAQQALREIGELIA 6209


>AE008876 AE006468 Salmonella typhimurium LT2, section 180 of 220 of
the complete genome.
Length = 22280

Score = 47.0 bits (110), Expect = 3e-07, Method: Composition-based stats.
Identities = 24/83 (28%), Positives = 42/83 (50%)
Frame = -1

Query: 1 MFQQEVTITAPNGLHTRPAAQFVKEAKGFTSEITVTSNGKSASAKSLFKLQTLGLTQGTV 60
M +Q+V + GLH RPAA K K + S +T+ +N K K + + G+ T+
Sbjct: 4406 MIRQKVRVMNSTGLHARPAATLAKIVKKYQSSLTLVNNDKEIPIKGMMSILGAGVKGNTM 4227

Query: 61 VTISAEGEDEQKAVEHLVKLMAE 83
+ + +G+DE ++ L A+
Sbjct: 4226 IDLICDGQDELAFMDELKSAFAD 4158


>AE008853 AE006468 Salmonella typhimurium LT2, section 157 of 220 of
the complete genome.
Length = 21918

Score = 40.8 bits (94), Expect = 3e-05, Method: Compositional matrix adjust.
Identities = 24/79 (30%), Positives = 42/79 (53%), Gaps = 1/79 (1%)
Frame = +2

Query: 3 QQEVTITAPNGLHTRPAAQFVKEAKGFTSEITVTSN-GKSASAKSLFKLQTLGLTQGTVV 61
+Q V +T G+H RPA + + +GF +E+ + ++ G A A S+ L L +G +
Sbjct: 1496 KQTVEVTNKLGMHARPAMKLFELMQGFDAEVLLRNDEGTEAEANSVIALLMLDSAKGRQI 1675

Query: 62 TISAEGEDEQKAVEHLVKL 80
I A G E +A+ ++ L
Sbjct: 1676 EIEATGPQEVEALAAVIAL 1732


>AE008892 AE006468 Salmonella typhimurium LT2, section 196 of 220 of
the complete genome.
Length = 20385

Score = 35.8 bits (81), Expect = 7e-04, Method: Composition-based stats.
Identities = 25/81 (30%), Positives = 35/81 (43%), Gaps = 2/81 (2%)
Frame = -1

Query: 5 EVTITAPNGLHTRPAAQFVKEAKGFTSEITVTS--NGKSASAKSLFKLQTLGLTQGTVVT 62
E T PNG+H RPA+ FTS+I + + SAKS L G
Sbjct: 4431 EFTCELPNGVHARPASHVETLCNTFTSQIEWHNLRTDRKGSAKSALALIGTDTLAGDHCQ 4252

Query: 63 ISAEGEDEQKAVEHLVKLMAE 83
+ G DEQ A + L + + +
Sbjct: 4251 LVISGADEQVACQRLSQWLRD 4189


>AE008703 AE006468 Salmonella typhimurium LT2, section 11 of 220 of
the complete genome.
Length = 20269

Score = 25.8 bits (55), Expect = 0.78, Method: Compositional matrix adjust.
Identities = 15/43 (34%), Positives = 24/43 (55%), Gaps = 2/43 (4%)
Frame = +2

Query: 21 QFVKEAKGFTSEITVTSNGKSASAKSLF--KLQTLGLTQGTVV 61
Q VKEA G+T +TV+S G S + + ++GL+Q +
Sbjct: 7109 QSVKEALGYTPGVTVSSRGASNTYDFVIIRGFSSVGLSQNNYL 7237


>AE008753 AE006468 Salmonella typhimurium LT2, section 57 of 220 of the
complete genome.
Length = 22182

Score = 23.1 bits (48), Expect = 5.4, Method: Composition-based stats.
Identities = 11/30 (36%), Positives = 16/30 (53%)
Frame = -3

Query: 17 RPAAQFVKEAKGFTSEITVTSNGKSASAKS 46
RP AQF A+GF +T+ + K +S
Sbjct: 20593 RPDAQFKNAARGFAEYVTLFTQRKDERQQS 20504


>AE008827 AE006468 Salmonella typhimurium LT2, section 131 of 220 of the
complete genome.
Length = 20396

Score = 22.7 bits (47), Expect = 5.8, Method: Composition-based stats.
Identities = 13/28 (46%), Positives = 17/28 (60%)
Frame = +3

Query: 53 LGLTQGTVVTISAEGEDEQKAVEHLVKL 80
L QG ++ ISAEG D+ E +VKL
Sbjct: 11418 LNRAQGALL-ISAEGIDDVAQAEEMVKL 11498


Database: st
Posted date: Nov 1, 2009 5:59 PM
Number of letters in database: 4,870,572
Number of sequences in database: 220

Lambda K H
0.309 0.123 0.318

Gapped
Lambda K H
0.267 0.0410 0.140


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 220
Number of Hits to DB: 267,329
Number of extensions: 2062
Number of successful extensions: 19
Number of sequences better than 10.0: 11
Number of HSP's gapped: 19
Number of HSP's successfully gapped: 11
Length of query: 85
Length of database: 1,623,524
Length adjustment: 60
Effective length of query: 25
Effective length of database: 1,610,324
Effective search space: 40258100
Effective search space used: 40258100
Neighboring words threshold: 13
Window for multiple hits: 40
X1: 16 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (21.8 bits)
S2: 43 (21.2 bits)