Документ взят из кэша поисковой машины. Адрес оригинального документа : http://kodomo.cmm.msu.ru/~nrar/Term4/Alignment.txt Дата изменения: Thu May 28 10:46:23 2009 Дата индексирования: Tue Oct 2 08:12:05 2012 Кодировка:

BLASTP 2.2.20+
Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro
A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and
David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new
generation of protein database search programs", Nucleic
Acids Res. 25:3389-3402.


Reference for compositional score matrix adjustment: Stephen
F. Altschul, John C. Wootton, E. Michael Gertz, Richa
Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and
Yi-Kuo Yu (2005) "Protein database searches using
compositionally adjusted substitution matrices", FEBS J.
272:5101-5109.


RID: 1WG7KMG611N


Query= ODP2_ECOLI P06959 RecName: Full=Dihydrolipoyllysine-residue
acetyltransferase component of pyruvate dehydrogenase complex;
EC=2.3.1.12; AltName: Full=E2; AltName: Full=Dihydrolipoamide
acetyltransferase component of pyruvate dehydrogenase complex;
Length=630


Score E
Sequences producing significant alignments: (Bits) Value

lcl|13849 ODP2_HUMAN P10515 RecName: Full=Dihydrolipoyllysine... 156 3e-42

ALIGNMENTS
>lcl|13849 ODP2_HUMAN P10515 RecName: Full=Dihydrolipoyllysine-residue acetyltransferase
component of pyruvate dehydrogenase complex,
mitochondrial; EC=2.3.1.12; AltName: Full=Pyruvate dehydrogenase
complex E2 subunit; Short=PDC-E2; Short=PDCE2; Short=E2;
AltName: Full=Dihydrolipoamide S-acetyltransferase component
of pyruvate dehydrogenase complex; AltName: Full=70 kDa
mitochondrial autoantigen of primary biliary cirrhosis; Short=PBC;
AltName: Full=M2 antigen complex 70 kDa subunit; Flags:
Precursor;
Length=647

Score = 156 bits (394), Expect = 3e-42, Method: Compositional matrix adjust.
Identities = 108/322 (33%), Positives = 172/322 (53%), Gaps = 28/322 (8%)

Query 296 APAPAAKAEAPAAAPAAKAEGKSEFAENDAYVHATPLIRRLAREFGVNLAKVKGTGRKGR 355
AP P+A A A P + V +PL ++LA E G++L +VKGTG GR
Sbjct 337 APTPSAPCPATPAGPKGR-------------VFVSPLAKKLAVEKGIDLTQVKGTGPDGR 383

Query 356 ILREDVQAYVKEAIKRAEAAPAATGGGIPGMLPWPKVDFSKFGEIEEVELGRIQKISGAN 415
I ++D+ ++V + A AA G PGM P P G ++ + I+++
Sbjct 384 ITKKDIDSFVPSKVAPAPAAVVPPTG--PGMAPVPT------GVFTDIPISNIRRVIAQR 435

Query 416 LSRNWVMIPHVTHFDKTDITELEAFRKQQNEEAAKRKLDVKITPVVFIMKAVAAALEQMP 475
L ++ IPH ++ E+ RK+ N+ R KI+ FI+KA A A ++P
Sbjct 436 LMQSKQTIPHYYLSIDVNMGEVLLVRKELNKILEGRS---KISVNDFIIKASALACLKVP 492

Query 476 RFNSSLSEDGQRLTLKKYINIGVAVDTPNGLVVPVFKDVNKKGIIELSRELMTISKKARD 535
NSS + R +++ VAV TP GL+ P+ + + KG+ ++ ++++++ KAR+
Sbjct 493 EANSSWMDTVIRQ--NHVVDVSVAVSTPAGLITPIVFNAHIKGVETIANDVVSLATKARE 550

Query 536 GKLTAGEMQGGCFTISSIGGLGTTHFAPIVNAPEVAIL--GVSKSAMEPVWNGKEFVPRL 593
GKL E QGG FTIS++G G +F+ I+N P+ IL G S+ + P N K F
Sbjct 551 GKLQPHEFQGGTFTISNLGMFGIKNFSAIINPPQACILAIGASEDKLVPADNEKGFDVAS 610

Query 594 MLPISLSFDHRVIDGADGARFI 615
M+ ++LS DHRV+DGA GA+++
Sbjct 611 MMSVTLSCDHRVVDGAVGAQWL 632


Score = 21.6 bits (44), Expect = 0.12, Method: Compositional matrix adjust.
Identities = 17/52 (32%), Positives = 27/52 (51%), Gaps = 1/52 (1%)

Query 23 KVGDKVEAEQSLITVEGDKASMEVPSPQAGIVKEIKVSVGDK-TQTGALIMI 73
K GDK+ + VE DKA++ S + + +I V+ G + GA+I I
Sbjct 114 KEGDKINEGDLIAEVETDKATVGFESLEECYMAKILVAEGTRDVPIGAIICI 165


Score = 21.2 bits (43), Expect = 0.15, Method: Compositional matrix adjust.
Identities = 21/75 (28%), Positives = 34/75 (45%), Gaps = 5/75 (6%)

Query 23 KVGDKVEAEQSLITVEGDKASMEVPSPQAGIVKEIKVSVGDK---TQTGALIMIFDSAD- 78
KVG+K+ L +E DKA++ + G + +I V G + T I++ AD
Sbjct 241 KVGEKLSEGDLLAEIETDKATIGFEVQEEGYLAKILVPEGTRDVPLGTPLCIIVEKEADI 300

Query 79 -GAADAAPAQAEEKK 92
AD P + + K
Sbjct 301 SAFADYRPTEVTDLK 315


Score = 20.8 bits (42), Expect = 0.22, Method: Compositional matrix adjust.
Identities = 9/21 (42%), Positives = 13/21 (61%), Gaps = 0/21 (0%)

Query 154 AGTVKEIKVNVGDKVSTGSLI 174
AGT+ + GDK++ G LI
Sbjct 105 AGTIARWEKKEGDKINEGDLI 125