ID   BIOA_ECOLI     STANDARD;      PRT;   429 AA.
AC   P12995;
DT   01-JAN-1990, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-1994, sequence version 2.
DT   07-FEB-2006, entry version 64.
DE   Adenosylmethionine-8-amino-7-oxononanoate aminotransferase
DE   (EC 2.6.1.62) (7,8-diamino-pelargonic acid aminotransferase) (DAPA
DE   aminotransferase).
GN   Name=bioA; OrderedLocusNames=b0774;
OS   Escherichia coli.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales;
OC   Enterobacteriaceae; Escherichia.
OX   NCBI_TaxID=562;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   MEDLINE=89066784; PubMed=3058702;
RA   Otsuka A.J., Buoncristiani M.R., Howard P.K., Flamm J., Johnson O.,
RA   Yamamoto R., Uchida K., Cook C., Ruppert J., Matsuzaki J.;
RT   "The Escherichia coli biotin biosynthetic enzyme sequences predicted
RT   from the nucleotide sequence of the bio operon.";
RL   J. Biol. Chem. 263:19577-19585(1988).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RA   Pearson B.M., McKee R.A.;
RT   "Genetic material for expression of biotin synthetase enzymes.";
RL   Patent number GB2216530, 11-OCT-1989.
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=K12 / MG1655;
RX   MEDLINE=97426617; PubMed=9278503; DOI=10.1126/science.277.5331.1453;
RA   Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V.,
RA   Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F.,
RA   Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J.,
RA   Mau B., Shao Y.;
RT   "The complete genome sequence of Escherichia coli K-12.";
RL   Science 277:1453-1474(1997).
RN   [4]
RP   X-RAY CRYSTALLOGRAPHY (1.8 ANGSTROMS).
RX   MEDLINE=99384134; PubMed=10452893; DOI=10.1006/jmbi.1999.2997;
RA   Kack H., Sandmark J., Gibson K., Schneider G., Lindqvist Y.;
RT   "Crystal structure of diaminopelargonic acid synthase: evolutionary
RT   relationships between pyridoxal-5'-phosphate-dependent enzymes.";
RL   J. Mol. Biol. 291:857-876(1999).
CC   -!- CATALYTIC ACTIVITY: S-adenosyl-L-methionine + 8-amino-7-
CC       oxononanoate = S-adenosyl-4-methylthio-2-oxobutanoate + 7,8-
CC       diaminononanoate.
CC   -!- COFACTOR: Pyridoxal phosphate.
CC   -!- PATHWAY: Cofactor biosynthesis; biotin biosynthesis; biotin from
CC       6-carboxyhexanoyl-CoA: step 2.
CC   -!- SUBUNIT: Homodimer.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm.
CC   -!- SIMILARITY: Belongs to the class-III pyridoxal-phosphate-dependent
CC       aminotransferase family.
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DR   EMBL; J04423; AAA23514.1; -; Genomic_DNA.
DR   EMBL; A11524; CAA00964.1; -; Unassigned_DNA.
DR   EMBL; U00096; AAC73861.1; -; Genomic_DNA.
DR   PIR; F64813; XNECDP.
DR   PDB; 1DTY; X-ray; A/B=1-429.
DR   PDB; 1MGV; X-ray; A/B=1-429.
DR   PDB; 1MLY; X-ray; A/B=1-429.
DR   PDB; 1MLZ; X-ray; A/B=1-429.
DR   PDB; 1QJ3; X-ray; A/B=1-429.
DR   PDB; 1QJ5; X-ray; A/B=1-429.
DR   PDB; 1S06; X-ray; A/B=1-429.
DR   PDB; 1S07; X-ray; A/B=1-429.
DR   PDB; 1S08; X-ray; A/B=1-429.
DR   PDB; 1S09; X-ray; A/B=1-429.
DR   PDB; 1S0A; X-ray; A/B=1-429.
DR   EchoBASE; EB0115; -.
DR   EcoGene; EG10117; bioA.
DR   BioCyc; EcoCyc:DAPASYN-MONOMER; -.
DR   GO; GO:0005515; F:protein binding; IPI.
DR   InterPro; IPR005814; Aminotrans_3.
DR   InterPro; IPR005815; BioA.
DR   PANTHER; PTHR11986; Aminotrans_3; 1.
DR   PANTHER; PTHR11986:SF14; BioA; 1.
DR   Pfam; PF00202; Aminotran_3; 1.
DR   TIGRFAMs; TIGR00508; bioA; 1.
DR   PROSITE; PS00600; AA_TRANSFER_CLASS_3; 1.
KW   3D-structure; Aminotransferase; Biotin biosynthesis;
KW   Complete proteome; Pyridoxal phosphate; Transferase.
FT   CHAIN         1    429       Adenosylmethionine-8-amino-7-oxononanoate
FT                                aminotransferase.
FT                                /FTId=PRO_0000120366.
FT   BINDING     274    274       Pyridoxal phosphate (covalent).
FT   CONFLICT     11     11       R -> P (in Ref. 2).
FT   CONFLICT     99    102       TPQP -> SGRNA (in Ref. 1).
FT   HELIX         3     12
FT   STRAND       13     13
FT   TURN         16     17
FT   STRAND       27     34
FT   TURN         35     35
FT   STRAND       36     39
FT   TURN         40     41
FT   STRAND       44     47
FT   TURN         50     54
FT   TURN         56     57
FT   STRAND       59     59
FT   HELIX        62     74
FT   STRAND       83     84
FT   HELIX        86     98
FT   TURN        101    102
FT   STRAND      103    109
FT   HELIX       112    129
FT   TURN        130    131
FT   STRAND      136    140
FT   TURN        141    142
FT   HELIX       149    152
FT   TURN        153    154
FT   TURN        157    161
FT   HELIX       162    165
FT   TURN        166    168
FT   STRAND      173    175
FT   STRAND      181    181
FT   HELIX       188    191
FT   HELIX       192    201
FT   TURN        202    204
FT   STRAND      205    210
FT   STRAND      214    215
FT   TURN        217    219
FT   STRAND      221    223
FT   TURN        225    225
FT   HELIX       226    238
FT   TURN        239    239
FT   STRAND      241    245
FT   TURN        247    254
FT   HELIX       259    263
FT   TURN        264    264
FT   STRAND      269    272
FT   HELIX       274    277
FT   TURN        278    279
FT   STRAND      284    289
FT   HELIX       290    297
FT   TURN        298    298
FT   TURN        300    301
FT   TURN        309    312
FT   HELIX       314    328
FT   TURN        329    329
FT   HELIX       331    347
FT   HELIX       348    352
FT   TURN        354    355
FT   STRAND      356    362
FT   TURN        363    364
FT   STRAND      365    370
FT   STRAND      374    374
FT   HELIX       376    385
FT   TURN        386    387
FT   STRAND      388    389
FT   STRAND      393    393
FT   TURN        394    395
FT   STRAND      396    399
FT   TURN        403    404
FT   HELIX       407    420
FT   TURN        421    421
FT   HELIX       424    426
FT   STRAND      427    427
SQ   SEQUENCE   429 AA;  47336 MW;  84D2D1AE3A1280FF CRC64;
     MTTDDLAFDQ RHIWHPYTSM TSPLPVYPVV SAEGCELILS DGRRLVDGMS SWWAAIHGYN
     HPQLNAAMKS QIDAMSHVMF GGITHAPAIE LCRKLVAMTP QPLECVFLAD SGSVAVEVAM
     KMALQYWQAK GEARQRFLTF RNGYHGDTFG AMSVCDPDNS MHSLWKGYLP ENLFAPAPQS
     RMDGEWDERD MVGFARLMAA HRHEIAAVII EPIVQGAGGM RMYHPEWLKR IRKICDREGI
     LLIADEIATG FGRTGKLFAC EHAEIAPDIL CLGKALTGGT MTLSATLTTR EVAETISNGE
     AGCFMHGPTF MGNPLACAAA NASLAILESG DWQQQVADIE VQLREQLAPA RDAEMVADVR
     VLGAIGVVET THPVNMAALQ KFFVEQGVWI RPFGKLIYLM PPYIILPQQL QRLTAAVNRA
     VQDETFFCQ
//