Документ взят из кэша поисковой машины. Адрес оригинального документа : http://kodomo.cmm.msu.ru/~igogo/Term3/KPN2JUN2003_5.txt Дата изменения: Fri Oct 6 19:20:20 2006 Дата индексирования: Tue Oct 2 12:13:40 2012 Кодировка:

BLASTP 2.2.10 [Oct-19-2004]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.

Query= KPN2Jun2003_5 [6778 - 7383]
(202 letters)

Database: 3ec
4855 sequences; 1,534,022 total letters

Searching..........done

Score E
Sequences producing significant alignments: (bits) Value

ACRE_ECOLI P24180 Acriflavine resistance protein E precursor (Pr... 85 4e-18
ACRA_ECOLI P0AE06 Acriflavine resistance protein A precursor. 80 1e-16
MDTE_ECOLI P37636 Multidrug resistance protein mdtE precursor. 74 1e-14
MDTA_ECOLI P76397 Multidrug resistance protein mdtA precursor (M... 64 6e-12
CUSB_ECOLI P77239 Cation efflux system protein cusB precursor. 43 2e-05

>ACRE_ECOLI P24180 Acriflavine resistance protein E precursor
(Protein envC).
Length = 385

Score = 84.7 bits (208), Expect = 4e-18
Identities = 61/202 (30%), Positives = 96/202 (47%), Gaps = 8/202 (3%)

Query: 2 ITSGNLVTAGDTASVLTTLVSQKTVYVYFDVDEST--YLHYQNLARRGQGASSDNQALPV 59
+T G LVT G T + T Q+ +Y DV +S+ ++ + +G +N V
Sbjct: 188 VTEGALVTNGQTTELATV---QQLDPIYVDVTQSSNDFMRLKQSVEQGN-LHKENATSNV 243

Query: 60 EIGLVGEEGYPHQGKVDFLDNQLTPSTGTIRMRALLDNSQRLFTPGLFARVRLPGSAEFK 119
E+ + + YP +G + F D + STG+I +RA+ N Q PG+F R R+ +
Sbjct: 244 ELVMENGQTYPLKGTLQFSDVTVDESTGSITLRAVFPNPQHTLLPGMFVRARIDEGVQPD 303

Query: 120 ATLIDDKAV-LTDQDRKYVYIVDKDGKAQRRDITPGRLADGLRIVQKGLNPGDSVIVDGL 178
A LI + V T + V IV+ + + R + + ++ +GL GD VIV GL
Sbjct: 304 AILIPQQGVSRTPRGDATVLIVNDKSQVEARPVVASQAIGDKWLISEGLKSGDQVIVSGL 363

Query: 179 QKVFMPGMPVNAKTVAMTSSAT 200
QK PG V A T +A+
Sbjct: 364 QKA-RPGEQVKATTDTPADTAS 384


>ACRA_ECOLI P0AE06 Acriflavine resistance protein A precursor.
Length = 397

Score = 80.1 bits (196), Expect = 1e-16
Identities = 57/195 (29%), Positives = 91/195 (46%), Gaps = 8/195 (4%)

Query: 2 ITSGNLVTAGDTASVLTTLVSQKTVYVYFDVDESTYLHYQNLARRGQGASSDNQALPVEI 61
+T G LV G A+ L T+ +YV + +L + G + +A ++
Sbjct: 189 VTEGALVQNGQ-ATALATVQQLDPIYVDVTQSSNDFLRLKQELANGTLKQENGKA---KV 244

Query: 62 GLVGEEG--YPHQGKVDFLDNQLTPSTGTIRMRALLDNSQRLFTPGLFARVRLPGSAEFK 119
L+ +G +P G ++F D + +TG+I +RA+ N PG+F R RL
Sbjct: 245 SLITSDGIKFPQDGTLEFSDVTVDQTTGSITLRAIFPNPDHTLLPGMFVRARLEEGLNPN 304

Query: 120 ATLIDDKAVL-TDQDRKYVYIVDKDGKAQRRDITPGRLADGLRIVQKGLNPGDSVIVDGL 178
A L+ + V T + V +V D K + R I + +V +GL GD V++ GL
Sbjct: 305 AILVPQQGVTRTPRGDATVLVVGADDKVETRPIVASQAIGDKWLVTEGLKAGDRVVISGL 364

Query: 179 QKVFMPGMPVNAKTV 193
QKV PG+ V A+ V
Sbjct: 365 QKV-RPGVQVKAQEV 378


>MDTE_ECOLI P37636 Multidrug resistance protein mdtE precursor.
Length = 385

Score = 73.6 bits (179), Expect = 1e-14
Identities = 54/202 (26%), Positives = 94/202 (46%), Gaps = 8/202 (3%)

Query: 2 ITSGNLVTAGDTASVLTTLVSQKTVYVYFDVDEST--YLHYQNLARRGQGASSDNQALPV 59
+T G LVTA S++T Q+ +Y D+ +S +L + GQ + PV
Sbjct: 185 VTVGALVTANQADSLVTV---QRLDPIYVDLTQSVQDFLRMKEEVASGQ-IKQVQGSTPV 240

Query: 60 EIGLVGEEGYPHQGKVDFLDNQLTPSTGTIRMRALLDNSQRLFTPGLFARVRLPGSAEFK 119
++ L + Y G + F D + +TG++ +RA+ N PG++ + +
Sbjct: 241 QLNLENGKRYSQTGTLKFSDPTVDETTGSVTLRAIFPNPNGDLLPGMYVTALVDEGSRQN 300

Query: 120 ATLIDDKAVLTD-QDRKYVYIVDKDGKAQRRDITPGRLADGLRIVQKGLNPGDSVIVDGL 178
L+ + V + Q + I+DKD Q R+I + +V GL GD VIV GL
Sbjct: 301 VLLVPQEGVTHNAQGKATALILDKDDVVQLREIEASKAIGDQWVVTSGLQAGDRVIVSGL 360

Query: 179 QKVFMPGMPVNAKTVAMTSSAT 200
Q++ PG+ A + + +++T
Sbjct: 361 QRI-RPGIKARAISSSQENAST 381


>MDTA_ECOLI P76397 Multidrug resistance protein mdtA precursor
(Multidrug transporter mdtA).
Length = 415

Score = 64.3 bits (155), Expect = 6e-12
Identities = 41/177 (23%), Positives = 81/177 (45%), Gaps = 8/177 (4%)

Query: 5 GNLVTAGDTASVLTTLVSQKTVYVYFDVDESTYLHYQNLARRGQGASSDNQALPVEIGLV 64
GN +++GDT ++ + + + F + ES + G+ L VE
Sbjct: 214 GNQISSGDTTGIVV-ITQTHPIDLVFTLPESDIATVVQAQKAGK-------PLVVEAWDR 265

Query: 65 GEEGYPHQGKVDFLDNQLTPSTGTIRMRALLDNSQRLFTPGLFARVRLPGSAEFKATLID 124
+G + LDNQ+ +TGTI+++A +N P F R+ E A +I
Sbjct: 266 TNSKKLSEGTLLSLDNQIDATTGTIKVKARFNNQDDALFPNQFVNARMLVDTEQNAVVIP 325

Query: 125 DKAVLTDQDRKYVYIVDKDGKAQRRDITPGRLADGLRIVQKGLNPGDSVIVDGLQKV 181
A+ + +V++++ + K + +TPG +++ G++ GD V+ DG+ ++
Sbjct: 326 TAALQMGNEGHFVWVLNSENKVSKHLVTPGIQDSQKVVIRAGISAGDRVVTDGIDRL 382


>CUSB_ECOLI P77239 Cation efflux system protein cusB precursor.
Length = 407

Score = 42.7 bits (99), Expect = 2e-05
Identities = 25/94 (26%), Positives = 50/94 (53%), Gaps = 1/94 (1%)

Query: 85 STGTIRMRALLDNSQRLFTPGLFARVRLPGSAEFKATLIDDKAVLTDQDRKYVYIVDKDG 144
+T T+++R +DN+ PG+ A ++L ++E LI +A++ + V VD DG
Sbjct: 290 ATRTLQLRLEVDNADEALKPGMNAWLQLNTASE-PMLLIPSQALIDTGSEQRVITVDADG 348

Query: 145 KAQRRDITPGRLADGLRIVQKGLNPGDSVIVDGL 178
+ + + + + G+ ++ GL G+ V+ GL
Sbjct: 349 RFVPKRVAVFQASQGVTALRSGLAEGEKVVSSGL 382


Database: 3ec
Posted date: Oct 6, 2006 3:21 AM
Number of letters in database: 1,534,022
Number of sequences in database: 4855

Lambda K H
0.317 0.135 0.377

Gapped
Lambda K H
0.267 0.0410 0.140


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 585,516
Number of Sequences: 4855
Number of extensions: 24265
Number of successful extensions: 85
Number of sequences better than 1.0e-03: 5
Number of HSP's better than 0.0 without gapping: 3
Number of HSP's successfully gapped in prelim test: 2
Number of HSP's that attempted gapping in prelim test: 79
Number of HSP's gapped (non-prelim): 5
length of query: 202
length of database: 1,534,022
effective HSP length: 80
effective length of query: 122
effective length of database: 1,145,622
effective search space: 139765884
effective search space used: 139765884
T: 11
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)
S2: 85 (37.4 bits)