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Дата изменения: Wed Dec 9 03:26:34 2009
Дата индексирования: Tue Oct 2 14:02:38 2012
Кодировка: Windows-1251
PurR sites

Экспериментально установленные сайты связывания белка PurR из Escherichia coli

                                                                                                                             
         >codB                                                                                                                             
         aaaaaatatatttccccacgaaaacgattgctttttatcttcagatgaatagaatgcggcggattttttgggtttcaaacagcaa                                      
         >purE                                                                                                                             
         tgatttcacagccacgcaaccgttttccttgctctctttccgtgctattctctgtgccctctaaagccgagagttgtgcaccaca                                      
         >pyrC                                                                                                                             
         agggcgcattcgcgccctttatttttcgtgcaaaggaaaacgtttccgcttatcctttgtgtccggcaaaaacatcccttcagcc                                      
         >purR                                                                                                                             
         ggcgtaccgcaacacttttgttgtgcgtaaggtgtgtaaaggcaaacgtttaccttgcgattttgcaggagctgaagttagggtc                                      
         >cvpA                                                                                                                             
         tttattgatgcgcgggaaggaaatccctacgcaaacgttttctttttctgttagaatgcgccccgaacaggatgacagggcgtaa                                      
         >purM                                                                                                                             
         aaaggttgtgtaaagcagtctcgcaaacgtttgctttccctgttagaattgcgccgaattttatttttctaccgcaagtaacgcg                                      
         >guaB                                                                                                                             
         gatagcaagcattttttgcaaaaaggggtagatgcaatcggttacgctctgtataatgccgcggcaatatttattaaccactctg                                      
         >glnB                                                                                                                             
         ttcccgacacgagctggatgcaaacgatttcaaggaatgaattggcgttatgtgttacgtttagcagatcaaaagacaggcgacc                                      
         >purL                                                                                                                             
         ttatttccacgcaaacggtttcgtcagcgcatcagattctttataatgacgcccgtttcccccccttgggtacaccgaaagctta                                      
         >purA                                                                                                                             
         aggtcatttttgagtgcaaaaagtgctgtaactctgaaaaagcgatggtagaatccatttttaagcaaacggtgattttgaaaaa                                      
         

Выданный мне вариант последовательности ДНК E.coli, находящейся перед генами, регулируемыми белком PurR с выделенными мотивами, которые были найденны программой MEME.

                                                                                                                             
         >codB                                                                                                                         
         catgctcagatgcctgatgcgacgctgacgcgtcttatcaggcctacccactgtttttacaccgataatttttcccccacctttttgcactcattcatat                          
         aaaaaatatatttccccacgaaaacgattgctttttatcttcagatgaatagaatgcggcggattttttgggtttcaaacagcaaaaagggggaatttcg                       
                                                                                                                        
         >purE<                                                                                                                         
         tggtactgggtgcctggcatacggaaggttcaatggtgaaagtcacggcggatgacgttgagctgattcattttccgttttaaaaaacccgcaactttgctg                        
         atttcacagccacgcaaccgttttccttgctctctttccgtgctattctctgtgccctctaaagccgagagttgtgcaccacaggagttttaagacgc                         
                                                                                                                      
         >pyrC                                                                                                                         
         gcgaagatggcagccagggcgaagcaatggcgaaaaaactggcgaaaggcattgaagtgaagccaggcgaaattgtcattccatttactgattaatcacgaggg                      
         cgcattcgcgccctttatttttcgtgcaaaggaaaacgtttccgcttatcctttgtgtccggcaaaaacatcccttcagccggagcatagagatta                           
                                                                                                                        
         >purR                                                                                                                         
         ttaccacttccccttttcgtcaagatcggccaaaattccacgcttacactatttgcgtactggccattgaccccttcctgacgctccgtgtcgtttttccg                         
         gcgtaccgcaacacttttgttgtgcgtaaggtgtgtaaaggcaaacgtttaccttgcgattttgcaggagctgaagttagggtctggagtgaaatggaa
                                                                                                                        
         >cvpA                                                                                                                         
         cgtaggtcggataaggcgtttacgccgcatccgacacgcattgcccgatgccgcaaaggcataaaaagtcgatggcgttgaatattttttcagcgccatttt                        
         tattgatgcgcgggaaggaaatccctacgcaaacgttttctttttctgttagaatgcgccccgaacaggatgacagggcgtaaaatcgtgggacacat                         
                                                                                                                           
         >purM                                                                                                                        
         atattcagatggttatgaaagaagattattccatccgaaaactaacctttaccctggcacaagtcttctttcgccgcgcgcctggggaaaagacgtgcaaaaag                      
         gttgtgtaaagcagtctcgcaaacgtttgctttccctgttagaattgcgccgaattttatttttctaccgcaagtaacgcgtggggacccaagcag                         
                                                                                                                       
         >guaB                                                                                                                         
         actaacggtaaaaattgcaggggattgagaaggtaacatgtgagcgagatcaaattctaaatcagcaggttattcagtcgatagtaacccgcccttcggggata                      
         gcaagcattttttgcaaaaaggggtagatgcaatcggttacgctctgtataatgccgcggcaatatttattaaccactctggtcgagatattgccc                           
                                                                                                                      
         >glnB                                                                                                                         
         aactcaactatttgcgtaagctgctgcaaatcaccaaaggcaacgtcacccacgcggcgagaatggcggggcgcaaccggacagaattttataaactgctttc                       
         ccgacacgagctggatgcaaacgatttcaaggaatgaattggcgttatgtgttacgtttagcagatcaaaagacaggcgaccttttcaaggaatagc                          
                                                                                                                        
         >purL                                                                                                                         
         aggtttactcataaatgagcggcattttgcgtaaacctgcgccagatggcaacttattacagccattggcggcacgcgttgctaattcacgatggtgattttat                      
         ttccacgcaaacggtttcgtcagcgcatcagattctttataatgacgcccgtttcccccccttgggtacaccgaaagcttagaagacgagagactt                           
                                                                                                                        
         >purA                                                                                                       
         accaatttgcccgataatattttacgtcgttttggcggtggacttgtggttgcgggcgttgtggtctactacatgttgaggaaaacgattggctgaac                     
         aaaaaacagactgatcgaggtcatttttgagtgcaaaaagtgctgtaactctgaaaaagcgatggtagaatccatttttaagcaaacggtgattttgaaaaa                            
                                                                                                                                           
         >carA                                                                                                                         
         tgtcgcttaatgcctgtaaaacatgcatgagccacaaaataatataaaaaatcccgccattaagttgacttttagcgcccatatctccagaatgccgccgtttgccag           
         aaattcgtcggtaagcagatttgcattgatttacgtcatcattgtgaattaatatgcaaataaagtgagtgaatattctctggagggtgttt