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21th Colloquium of the Association of French speaking diatomists (ADLaF)
Nantes (France), September 10-13, 2002
The colloquium will take place in the Museum of natural History, in the
city centre. Participants are invited to present oral communications or
posters (both in French) dealing with fossil and recent diatoms from
freshwater, marine and brackish environments, taxonomy, ecology, water
quality,... Statutory assembly of the association will be held on Thursday
September12th in the late afternoon. Wednesday 11th will be partly devoted
to an excursion to the Loire estuary area. Registration and deadline for
abstracts of the oral communications and posters: June 28, 2002. Abstracts
are to be sent to Dr Yves RINCE / ISOMer / 2, rue de la Houssiniиre, B.P.
92208, F-44322 Nantes cedex 03, France.
The conference registration fee is 50 euro. Information can be obtained
at the association's web site: http://perso.club-
internet.fr/clci/21emeColloqueADLaF.HTM
http://perso.club-internet.fr/clci/diatom-ADLaF.htm or by e-mailing to the
following address: Didier.GUILLARD@pays-de-la-loire.environnement.gouv.fr
Secretariat and organisation of the colloquium:
Didier GUILLARD, Maxence NAZART
DIREN-SEMA Pays de la Loire
12, rue Menou
F-44035-NANTES cedex
France
E-mail: Didier.GUILLARD@pays-de-la-loire.environnement.gouv.fr
Source: http://www.seaweed.ie/algae-l/default.html


К 250-летию Московского Государственного Университета им. М.В. Ломоносова
Одиннадцатое Международное совещание по филогении растений
28-31 января 2003
1е Информационное письмо
Дорогие коллеги!
Приглашаем Вас принять участие в работе Одиннадцатого Международного
совещания по филогении растений, которое будет проходить 28 -31 января 2003
г. на Биологическом факультете МГУ.
Председатель Оргкомитета:
Декан Биологического факультета МГУ,
заслуженный профессор МГУ М.В. Гусев
Оргкомитет:
член-корр. РАН, проф. В.Н. Павлов,
проф. Ю.Т. Дьяков,
проф. В.С. Новиков,
д.б.н. А.К. Тимонин
Секретарь Оргкомитета:
М.Е.Площинская

Предполагается обсуждение широкого круга вопросов по следующим основным
направлениям:
- Филогения разных групп грибов, высших и низших растений.
- Основные особенности микро- и макроэволюции растений и грибов.
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Для участия в совещании необходимо до 15 сентября 2002 г. представить:
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2. Просим представлять не более двух докладов от одного лица (второй - в
соавторстве).
3. Тезисы доклада:
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город.
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65 знаков).
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Заявки и тезисы могут быть высланы по почте, по e-mail, или переданы лично.
Оргкомитет уведомит Вас относительно принятия или отклонения Ваших тезисов.
Оргвзнос ориентировочно составит 100 рублей (организационные расходы) с
каждого участника, и 150 руб. за публикацию каждых тезисов (сумма зависит
от числа тезисов, а не от числа авторов).
Адрес Оргкомитета:
119 992, Москва, Ленинские горы,
Московский государственный университет,
Биологический ф-т, каф. высших растений;
Площинская Мария Евгеньевна
Тел: (095)939 16 03
e-mail: ploshinskaya@herba.msu.ru
Предпочтительной формой связи с Оргкомитетом является электронная почта.
Мы будем очень признательны, если Вы сообщите эту информацию Вашим
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С уважением,
ОРГКОМИТЕТ
Source: http://herba.msu.ru

|НАУКОВА ПЕР?ОДИКА / НАУЧНАЯ ПЕРИОДИКА / SCIENTIFIC PERIODICITY |

JOURNAL OF PHYCOLOGY
April 2002, Volume 38, Issue 2
Algae - Highlights:
Russell L. Chapman and Debra A. Waters. Green algae and land plants-An
answer at last? J. Phycol. 2002 38: 237-240.
Ecology and Population Biology:
Helena Guasch, Maria Paulsson, and Sergi Sabater. Effect of copper on algal
communities from oligotrophic calcareous streams. J. Phycol. 2002 38: 241-
248.
Two sets of experiments were done to quantify the effects of chronic
copper exposure on natural peri- phyton in a nonpolluted calcareous river.
The results of short-term (up to 6 h exposure) experiments corroborated the
significance of pH on copper toxicity. Copper toxicity increased when pH
was reduced from 8.6 to 7.7, and this was related to the effect of pH on
copper speciation (free copper concentration increased from 0.2% to 2.3% of
total copper). Longer term experiments demonstrated that periphyton
communities exposed to copper under pH variation (8.2-8.6) were already
affected at 10 µgћL-1 (20-80 ngћL-1 Cu2+) after 12 days of exposure. Copper
exposure caused stronger effects on structural (algal biomass and community
structure) than on functional (photosynthetic efficiency) parameters of
peri- phyton. Changes in community composition included the enhancement of
some taxa (Gomphonema gracile), the inhibition of others (Fragilaria
capucina and Phormidium sp.), and the appearance of filament malformations
(Mougeotia sp.). The results of our study demonstrated that several weeks
of exposure to copper (10-20 µgћL-1) were sufficient to cause chronic
changes in the periphyton of oligotrophic calcareous rivers. This degree of
copper pollution can be commonly found in the Mediterranean region as a
result of agricultural practices and farming activities. SUMMARY.

Kathleen M. RЭhland and John P. Smol. Freshwater diatoms from the Canadian
Arctic treeline and development of paleolimnological inference models. J.
Phycol. 2002 38: 249-264.
Relationships between surface sediment diatom assemblages and measured
environmental variables from 77 lakes in the central Canadian arctic
treeline region were examined using multivariate statistical methods. Lakes
were distributed across the arctic treeline from boreal forest to arctic
tundra ecozones, along steep climatic and environmental gradients. Forward
selection in canonical correspondence analysis determined that dissolved
inorganic carbon (DIC), dissolved organic carbon (DOC), total nitrogen
(TN), lake surface area, silica, lake-water depth, and iron explained
significant portions of diatom species variation. Weighted-averaging (WA)
regression and calibration techniques were used to develop inference models
for DIC, DOC, and TN from the estimated optima of the diatom taxa to these
environmental variables. Simple WA models with classical deshrinking
produced models with the strongest predictive abilities for all three
variables based on the bootstrapped root mean squared errors of prediction
(RMSEP). WA partial least squares showed little improvement over the
simpler WA models as judged by the jackknifed RMSEP. These models suggest
that it is possible to infer trends in DIC, DOC, and TN from fossil diatom
assemblages from suitably chosen lakes in the central Canadian arctic
treeline region. SUMMARY.

Pauli Snoeijs, Svenja Busse, and Marina Potapova. The importance of diatom
cell size in community analysis. J. Phycol. 2002 38: 265-281.
The large variation in size and shape in diatoms is shown by
morphometric measurements of 515 benthic and pelagic diatom species from
the Baltic Sea area. The largest mean cell dimension (mostly the apical
axis) varied between 4.2 and 653 µm, cell surface area between 55 and
344,000 µm2, and cell volume between 21 and 14.2 x 106 µm3. The shape-
related index, length to width ratio, was between 1.0 and 63.3 and the
shape- and size-related index, surface area to volume ratio, was between
0.02 and 3.13. Diatom community analysis by multivariate statistics is
usually based on counts of a fixed number of diatom valves with species
scores irrespective of cell size. This procedure underestimates the large
species for two reasons. First, the importance of a species with higher
cell volume is usually larger in a community. Second, larger species
usually have lower abundances and their occurrence in the diatom counts is
stochastic. This article shows that co-occurring small and large diatom
species can respond very differently to environmental constraints. Large
epiphytic diatoms responded most to macroalgal host species and small
epiphytic diatoms most to environmental conditions at the sampling site.
Large epilithic diatoms responded strongly to salinity, whereas small
epilithic diatoms did so less clearly. The conclusion is that different
scale-dependent responses are possible within one data set. The results
from the test data also show that important ecological information from
diatom data can be missed when the large species are neglected or
underestimated. SUMMARY.

Rodney D. Roberts, Michael KЭhl, Ronnie NЬhr Glud, and SЬren Rysgaard.
Primary production of crustose coralline red algae in a high arctic fjord.
J. Phycol. 2002 38: 273-283.
Crustose coralline algae occupied ~ 1%-2% (occasionally up to 7%) of
the sea floor within their depth range of 15-50 m, and they were the
dominant encrusting organisms and macroalgae beyond 20 m depth in Young
Sound, NE Greenland. In the laboratory, oxygen microelectrodes were used to
measure net photosynthesis (P) versus downwelling irradiance (Ed) and
season for the two dominant corallines [Phymatolithon foecundum (Kjellman)
DЭwel et Wegeberg 1996 and Phymatolithon tenue (Rosenvinge) DЭwel et
Wegeberg 1996] representing > 90% of coralline cover. Differences in P-Ed
curves between the two species, the ice-covered and open-water seasons, or
between specimens from 17 and 36 m depth were insignificant. The corallines
were low light adapted, with compensation irradiances (Ec) averaging 0.7-
1.8 µmol photonsћm-2ћs-1 and light adaptation (Ek) indices averaging 7-17
µmol photonsћm-2ћs-1. Slight photoinhibition was evident in most plants at
irradiances up to 160 µmol photonsћm-2ћs-1. Photosynthetic capacity (Pm)
was low, averaging 43-67 mmol O2ћm-2 thallusћd-1 (~ 250-400 g Cћm-2
thallusћyr-1). Dark respiration rates averaged ~ 5 mmol O2ћm-2 thallusћd-1.
In ice covered periods, Ed at 20 m depth averaged ~ 1 µmol photonsћm-2ћs-1,
with daily maxima of 2-3 µmol photonsћm-2ћs-1. During the open water
season, Ed at 20 m depth averaged ~ 7 µmol photonsћm-2ћs-1 with daily
maxima of ~ 30 µmol photonsћm-2ћs-1. Significant net primary production of
corallines was apparently limited to the 2-3 months with open water, and
the small contribution of corallines to primary production seems due to low
Pm values, low in situ irradiance, and their relatively low abundance in
Young Sound. SUMMARY.

Britas Klemens Eriksson, Gustav Johansson, and Pauli Snoeijs. Long-term
changes in the macroalgal vegetation of the inner Gullmar Fjord Swedish
Skagerrak coast. J. Phycol. 2002 38: 284-296.
We examined long-term changes in the macroalgal vegetation at Stora
BornЖ Island in the inner Gullmar Fjord on the Swedish Skagerrak coast.
This was made possible by access to a 1941 diving investigation. The same
sites were reinvestigated in 1998. Community composition and depth
distributions of species were compared and changes were analyzed with focus
on functional groups (size, thallus shape, and life-history traits). We
discovered a significant decrease in the depth extension of macroalgal
species and a dramatic decline of species richness in the lower littoral
(below 16 m of depth) compared with 57 years earlier. Ordination analysis
revealed that there was a significant difference in the community
composition between the two study periods. In general, small (<10 cm),
thin, filamentous, and aseasonal ephemerals increased in relative
abundance, whereas larger (>10 cm), coarsely branched, and perennial algae
decreased. Calibrations of individual species to local sediment cover,
using canonical correspondence analysis, indicated that part of the change
in species composition was related to sediment load. Furthermore, large-
scale climate differences (NAO Winter Index) between the study periods
indicated a higher impact of Baltic Sea and Kattegat water in the nutrient
dynamics of the fjord in the 1998 study. We concluded that the observed
long-term changes in the macroalgal community at Stora BornЖ Island were
consistent with an increased nutrient availability. SUMMARY.

Physiology and Biochemistry:
Yukie Yoshii, Shinichi Takaichi, Takashi Maoka, Satoshi Hanada, and Isao
Inouye. Characterization of two unique carotenoid fatty acid esters from
Pterosperma cristatum (Prasinophyceae, Chlorophyta). J. Phycol. 2002 38:
297-303.
Carotenoid composition and spectroscopic characteristics were analyzed
for Pterosperma cristatum Schiller, one of the most primitive known members
of green algae. This alga contained a substantial amount of carotenoid
esters, siphonaxanthin C14:1 trans-?2 ester and 6'-OH siphonaxanthin C14:1
trans-?2 ester, but lacked lutein. This is the first report of carotenoid
C14:1 trans-?2 esters from phototrophic organisms. In vivo absorption
spectra and excitation spectra of the cells revealed that these carotenoids
absorbed blue-green light and could transfer energy to chl a. These
carotenoids were concluded to function as antenna pigments in P. cristatum.
SUMMARY.

Myung-Soo Han, Young-Pil Kim, and Rose Ann Cattolico. Heterosigma akashiwo
(Raphidophyceae) resting cell formation in batch culture: strain identity
versus physiological response. J. Phycol. 2002 38: 304-317.
In this study, we examined the impact of environmental perturbation on
the movement of the toxic bloom-forming alga Heterosigma akashiwo (Hada)
Hada ex Y. Hara et Chihara [syn. H. carterae (Hulburt) F.J.R. Taylor]
between vegetative and resting cell phases of the life history. Resting
state induction, in batch culture, was most effective when vegetative cells
were subjected to low temperature (10њ C) and darkness for extended time
periods. Heterosigma cells in stasis had neither a cell wall nor scales but
were surrounded by a calyx, most probably of polysaccharide composition.
The resting cell was completely immobile, although both flagella remained
attached. Heterosigma resting cells did not require a maturation period
before successful activation to the vegetative state could occur. Cell
division and motility were impacted sequentially during both the induction
and activation phases of resting cell development. Our data show that
Heterosigma had an obligate light requirement for resting cell activation.
In replete medium, very low light fluences of 5 µmol photonsћm-2ћs-1 were
as effective as 60 µmol photonsћm-2ћs-1 in the initiation of activation.
Such sensitivity to extremely low light might give Heterosigma a
competitive advantage for bloom formation in nature. Reduced nitrate levels
significantly shortened the temporal transition of vegetative cells into
the resting cell phase of the life history. Additionally, when resting
cells induced in nitrate-limited medium were activated under nitrate-
replete condition, the efficiency of the activation response was directly
correlated to light availability. Both vegetative and resting cells
maintained a haploid DNA complement. Rapid amplified polymorphic DNA (RAPD)
analysis demonstrated variation in genetic identity among axenic
Heterosigma strains. Strain identity influenced success in resting cell
induction and survival in stasis. To date, no defined sexual cycle has been
described. These observations are discussed in terms of population fitness.
The data presented in this report provide a model algal system wherein the
molecular events that govern long-term stasis in an obligately autotrophic
organism can now be assessed. SUMMARY.

Shinya Yoshikawa, Chikako Nagasato, Yumiko Makino, Akio Murakami, Hiroshi
Kawai, Terunobu Ichimura, and Taizo Motomura. Nuclear histone proteins of
gametes in an oogamous and two isogamous brown algae. J. Phycol. 2002 38:
318-324.
Nuclear basic proteins (histones) were studied in male and female
gametes of the isogamous brown algae, Colpomenia bullosa (Saunders) Yamada
and Analipus japonicus (Harvey) Wynne and sperm of the oogamous Cystoseira
hakodatensis (Yendo) Fensholt by using SDS- and AUT-PAGE. Four major core
histones and several linker histone H1s were detected by electrophoresis.
Each of the core histones was identified by amino acid sequence analysis
and peptide mapping. Electrophoresis patterns of histones were the same in
male and female gametes and quite similar between the two species. The
composition of histone H1s in conspicuously condensed sperm nuclei of C.
hakodatensis was different from that in isogamous gametes. Electrophoresis
after micrococcal nuclease digestion of chromatin in male and female gamete
nuclei of C. bullosa and A. japonicus and sperm of C. hakodatensis resulted
in regular ladder patterns of DNA fragments (ca. 200 base pair). The
chromatin of the brown algal gametes thus has the typical nucleosome
structure. These results showed that chromatin condensation in sperm nuclei
of C. hakodatensis was associated with a modification of linker histone H1
but not by change of core histones, replacement by other basic proteins,
changes of repeating patterns, or disappearance of nucleosomes. SUMMARY.

Mirash Zhekisheva, Sammy Boussiba, Inna Khozin-Goldberg, Aliza Zarka, and
Zvi Cohen. Accumulation of oleic acid in Haematococcus pluvialis
(Chlorophyceae) under nitrogen starvation or high light is correlated with
that of astaxanthin esters. J. Phycol. 2002 38: 325-331.
The chlorophyte Haematococcus pluvialis accumulates large quantities of
astaxanthin under stress conditions. Under either nitrogen starvation or
high light, the production of each picogram of astaxanthin was accompanied
by that of 5 or 3-4 pg of fatty acids, respectively. In both cases, the
newly formed fatty acids, consisting mostly of oleic (up to 34% of fatty
acids in comparison with 13% in the control), palmitic, and linoleic acids,
were deposited mostly in triacylglycerols. Furthermore, the enhanced
accumulation of oleic acid was linearily correlated with that of
astaxanthin. Astaxanthin, which is mostly monoesterified, is deposited in
globules made of triacylglycerols. We suggest that the production of oleic
acid-rich triacylglycerols on the one hand and the esterification of
astaxanthin on the other hand enable the oil globules to maintain the high
content of astaxanthin esters. SUMMARY.

Ana T. Lombardi, Armando A. H. Vieira, and Luiz A. Sartori. Mucilaginous
capsule adsorption and intracellular uptake of copper by Kirchneriella
aperta (Chlorococcales). J. Phycol. 2002 38: 332-337.
The purpose of the present investigation was to evaluate possible
ecological and physiological functions of mucilaginous capsules produced by
the freshwater algae Kirchneriella aperta Teiling (Chlorococcales) as
related to copper ions. All experiments were performed using synthetic
media under laboratory-controlled conditions. Copper interactions were
investigated by distinguishing between adsorption onto the mucilaginous
material present at the surface of the cells, intracellular uptake, and
differentiation between total dissolved copper and free copper ions in the
culture medium. Kirchneriella aperta is sensitive to copper, as revealed by
a 96-h EC50 value of 10-9.22 M Cu2+. We demonstrated that the mucilaginous
capsules were able to sequester copper ions from the medium through a
passive mechanism, thus providing the cell with a mechanism able to
postpone the toxic effects of copper. The organic material that diffuses
into the test medium as well as the mucilaginous capsules produced by K.
aperta both effectively complex copper; thus, toxicity must be related to
free copper ions and not the total dissolved copper concentration in the
medium. SUMMARY.

Runar Thyrhaug, Aud Larsen, Corina P. D. Brussaard, and Gunnar Bratbak.
Cell cycle dependent virus production in marine phytoplankton. J. Phycol.
2002 38: 338-343.
In this study we investigated virus production in two marine
phytoplankton species and how it relates to the host's cell cycle.
Phaeocystis pouchetii (Hariot) Lagerheim and Pyramimonas orientalis
McFadden, Hill & Wetherby, growing synchronously in batch cultures, were
infected with their respective viruses (PpV and PoV) at four different
stages in the cell cycle and the production of free virus was then measured
for 30 h. The virus production in P. orientalis infected with PoV depended
on the time of infection, whereas no such relation was found for P.
pouchetii infected with PpV. The P. orientalis cultures infected at the end
of the dark period and at the beginning of the light period produced three
times more virus than those infected in the middle of the light period and
eight times more virus than those infected at the beginning of the dark
period. The latent periods for PpV and PoV were 12-14 h and 18-20 h,
respectively, and in both cases were independent of the host cell cycle.
The differences in virus production may be attributed to light or cell
cycle dependent regulation of host infection, metabolism, or burst size.
Regardless of the mechanism, these differences may be related to
differences in the ecological strategies of the hosts and their ability to
form blooms. SUMMARY.

JosИ Manuel Estevez, Marina Ciancia, and Alberto SaЗl Cerezo. Carrageenans
biosynthesized by carposporophytes of red seaweeds Gigartina skottsbergii
(Gigartinaceae) and Gymnogongrus torulosus (Phyllophoraceae). J. Phycol.
2002 38: 344-350.
Carrageenans biosynthesized by gametophytic and tetrasporic plants of
seaweeds belonging to the Gigartinaceae and Phyllophoraceae are different:
gametophytes produce carrageenans of the kappa family, whereas lambda-
carrageenans are extracted from tetrasporophytes. For Gigartina
skottsbergii Setchell and Gardner and Gymnogongrus torulosus Hooker et
Harvey, mature cystocarps were isolated and carrageenans were extracted.
Structural determination by methylation analysis, Fourier transform
infrared spectroscopy, and 13C-NMR spectroscopy showed that they were
kappa/iota-carrageenans. For the extract obtained from cystocarps of
Gigartina skottsbergii with water at room temperature, the ratio kappa:iota
was 1:0.30 and at 90њ C was 1:0.43; significant amounts of precursors were
also present. The extract obtained from cystocarps of Gymnogongrus
torulosus at 90њ C showed prevalence of iota-carrageenans (ratio kappa:iota
1:1.21). These extracts are similar to the polysaccharides produced by
gametophytes of these seaweeds. For Gigartina skottsbergii, it was possible
to separate the pericarpic tissue from the carposporophyte. Thus, they were
extracted separately, and the carrageenans isolated were studied as
described before, obtaining similar conclusions. These results clearly show
that whereas the carposporophytes are located inside the cystocarp, they
produce carrageenans of the kappa family despite of being diploid cells.
SUMMARY.

Cellular and Molecular Biology:
Allison M. L. van de Meene and Jeremy D. Pickett-Heaps. Valve morphogenesis
in the centric diatom Proboscia alata Sundstrom. J. Phycol. 2002 38: 351-
363.
Valve morphogenesis in Proboscia alata Sundstrom was followed in living
cells and during treatment with antiactin and antimicrotubule drugs. Once
cleaved, sibling cells rounded up and retracted. Soon, a granular
organizing center (OC) appeared adjacent to the stub of the initiated
valve. Silicification started within a silicon deposition vesicle (SDV)
adjacent to the OC. The elongating valve was initially tubular and sealed
at one end, creating the proboscis of the conical valve. The edge of the
SDV and thinnest region of the forming valve was lined by a sleeve of
bundled microtubules (MTs) that terminated short of the older more rigid
part of the valve. The growing proboscis of living cells treated with the
anti-MT drug oryzalin became grossly distorted. EM revealed dense material
lining the growing edge of the SDV; immunofluorescence microscopy showed a
ring of actin here. Applied to living cells, the antiactin drug
cytochalasin D caused the very young proboscis to collapse; in older
valves, the base of the proboscis expanded. Thus, valve morphogenesis
appeared controlled by the MT cytoskeleton, keeping the proboscis straight
while actin molded its conical outline. At the tip of the proboscis was a
slit resembling a labiate process. Its morphogenesis involved striated
fibers and two MTs, reminiscent of the fibers and MTs associated with raphe
morphogenesis. In contrast to spine-like processes that elongate by tip
growth, the tip of the proboscis was formed first, and the consequent
"antitip growth" suggests the tip was originally the center of the valve
face. SUMMARY.

Phylogenetics and Taxonomy:
Monique Turmel, Megumi Ehara, Christian Otis, and Claude Lemieux.
Phylogenetic relationships among streptophytes as inferred from chloroplast
small and large subunit rRNA gene sequences. J. Phycol. 2002 38: 364-375.
To gain insights into the phylogeny of charophytes and into their
relationships with other green algae and bryophytes, we analyzed the
chloroplast small and large subunit rRNA sequences of charophytes belonging
to five orders (Charales, Coleochaetales, Desmidiales, Klebsormidiales, and
Zygnematales), of chlorophytes from the four remaining classes of green
algae, and of bryophytes representing the three classes reported in this
group of land plants. We also probed the gene organization and intron
content of the chloroplast rDNA operon in charophytes and bryophytes. The
organization of this operon proved to be highly conserved, except in
members of the Desmidiales and Zygnematales. Homologous group II introns
were identified in the trnA(UGC) gene of all charophyte groups examined and
in the trnI(GAU) gene of charophytes from all orders except the Desmidiales
and Zygnematales. Phylogenetic analyses of concatenated rDNA sequences
consistently placed the prasinophyte Mesostigma viride Lauterborn at the
base of the Streptophyta and Chlorophyta, although alternative topologies
positioning Mesostigma within the Streptophyta could not be rejected. A
sister group relationship was unambiguously established between
Chaetosphaeridium globosum (Nordstedt) Klebahn and members of the
Coleochaetales. The Charales, Coleochaetales, Desmidiales, and Zygnematales
were found to be monophyletic, and a sister group relationship was observed
for the Desmidiales and Zygnematales. Although our analyses failed to
resolve the branching order of the Coleochaetales, Charales,
Desmidiales/Zygnematales, and bryophytes, they revealed that the
problematic charophyte taxon Entransia fimbriata Hughes strongly clusters
with Klebsormidium flaccidum (KЭtzing) Silva, Mattox et Blackwell to form a
basal lineage relative to the other charophyte orders examined. SUMMARY.

Mark A. Buchheim, Julie A. Buchheim, Tracy Carlson, and Paul Kugrens.
Phylogeny of Lobocharacium (Chlorophyceae) and allies: A study of 18S and
26S rDNA data. J. Phycol. 2002 38: 376-383.
The phylogenetic affinities of Lobocharacium coloradoense were
investigated by analysis of combined 18S and 26S rDNA data. Results from
both parsimony and likelihood methods supported a close alliance among
Lobocharacium, Characiosiphon, and Characiochloris. These three taxa formed
a clade near the base of the "Dunaliella" group within the chlamydomonad
lineage. Protosiphon, which exhibits a siphonous habit similar to
Characiosiphon and Lobocharacium, was not resolved as a close ally of the
latter two taxa. The Lobocharacium alliance was characterized by the
presence of an attachment pad associated with the nonmotile vegetative
stage and pyrenoids that possess cytoplasmic invaginations. The pyrenoid
feature is an ultrastructural trait that has now been observed in five
different chlorophycean lineages. The Lobocharacium-Characiosiphon-
Characiochloris clade is not predicted by any classifications of green
algae. Additional taxon and data sampling need to be completed to resolve
inconsistencies between the molecular phylogenetic evidence and at least
some of the current family-level taxa. SUMMARY.

Denis Baurain, Laurent Renquin, Stana Grubisic, Patsy Scheldeman, Amha
Belay, and Annick Wilmotte. Remarkable conservation of internally
transcribed spacer sequences of Arthrospira ("Spirulina") (Cyanophyceae,
Cyanobacteria) strains from four continents and of recent and 30-year-old
dried samples from Africa. J. Phycol. 2002 38: 384-393.
The internally transcribed spacer (ITS) sequences of 21 Arthrospira
clonal strains from four continents and assigned to four different species
(A. platensis, A. maxima, A. fusiformis, A. indica) in the culture
collections were determined. Two main clusters, I and II, were
differentiated by 49 positions out of 475 nt or 477 nt, respectively. Each
cluster was further subdivided into two subclusters. Subclusters I.A and
I.B were separated by two substitutions, whereas subclusters II.A and II.B
were distinguished by four substitutions. After direct sequencing of the
PCR products, three dried samples from Chad aged between 3 months and 35
years yielded a sequence belonging to subcluster I.A, as did a recent
commercial product. The strains grown in production plants belonged to the
same (sub)clusters as strains from culture collections, mainly I.A and II.
PCR primers specific for each cluster and subcluster were designed and
tested with crude cell lysates of Arthrospira strains. One dried sample
("dihИ" 1) and a herbarium sample from Lake Sonachi (Kenya) only contained
I.A sequences, whereas the commercial product was a mixture of the four
genotypes and the other two dried samples contained minor polymorphisms
characteristic of different clusters. Five clonal Arthrospira strains,
thought to be duplicates, showed the simultaneous presence of the two forms
of the four diagnostic positions that distinguish subclusters genotype II.A
and genotype II.B. This is likely to be caused by multiple copies of the
rDNA operon, in a intermediate stage of homogenization between subcluster
II.A and subcluster II.B. The high conservation of ITS sequences is in
contrast with the assignment to four different species, the great
morphological variability of the strains, and their wide geographic
distribution. SUMMARY.

Charles F. Delwiche, Kenneth G. Karol, Matthew T. Cimino, and Kenneth J.
Sytsma. Phylogeny of the genus Coleochaete (Coleochaetales, Charophyta) and
related taxa inferred by analysis of the chloroplast gene rbcL. J. Phycol.
2002 38: 394-403.
The genus Coleochaete BrИb. is considered to be a key taxon in the
evolution of green algae and embryophytes (land plants), but only a few of
the approximately 15 species have been studied with molecular phylogenetic
methods. We report here the sequences of the gene rbcL from six new
cultures of Coleochaete and two of Chaetosphaeridium Klebahn. These
sequences were combined with 32 additional sequences, and phylogenetic
analyses were performed with maximum likelihood, distance optimality, and
parsimony methods. Important subgroups within Coleochaete include two
primary lineages, one marked by fully corticated zygotes and the other by
naked or weakly corticated zygotes. In the first lineage there is a
subclade with tightly joined filaments and distinctive ("T-shaped") cell
division, an assemblage of strains that resembles the endophytic species
Coleochaete nitellarum Jost, and a clade with loosely joined filaments and
"Y-shaped" cell divisions. Consistent with recent multigene phylogenies,
these analyses support the monophyly of the Coleochaetales, place the
Charales as the sister taxon to land plants, and indicate that
Chaetosphaeridium is far more closely related to Coleochaete than to
Mesostigma Lauterborn. SUMMARY.

Techniques:
Isabelle C. Biegala, Gabrielle Kennaway, Elsa Alverca, Jean-FranГois
Lennon, Daniel Vaulot, and Nathalie Simon. Identification of bacteria
associated with dinoflagellates (Dinophyceae) Alexandrium spp. using
tyramide signal amplification-fluorescent in situ hybridization and
confocal microscopy. J. Phycol. 2002 38: 404-411.
In the marine environment, phytoplankton and bacterioplankton can be
physically associated. Such association has recently been hypothesized to
be involved in the toxicity of the dinoflagellate genus Alexandrium.
However, the methods, which have been used so far to identify, localize,
and quantify bacteria associated with phytoplankton, are either
destructive, time consuming, or lack precision. In the present study we
combined tyramide signal amplification-fluorescent in situ hybridization
(TSA-FISH) with confocal microscopy to determine the physical association
of dinoflagellate cells with bacteria. Dinoflagellate attached microflora
was successfully identified with TSA-FISH, whereas FISH using monolabeled
probes failed to detect bacteria, because of the dinoflagellate
autofluorescence. Bacteria attached to entire dinoflagellates were further
localized and distinguished from those attached to empty theca, by using
calcofluor and DAPI, two fluorochromes that stain dinoflagellate theca and
DNA, respectively. The contribution of specific bacterial taxa of attached
microflora was assessed by double hybridization. Endocytoplasmic and
endonuclear bacteria were successfully identified in the nonthecate
dinoflagellate Gyrodinium instriatum. In contrast, intracellular bacteria
were not observed in either toxic or nontoxic strains of Alexandrium spp.
Finally, the method was successfully tested on natural phytoplankton
assemblages, suggesting that this combination of techniques could prove a
useful tool for the simultaneous identification, localization, and
quantification of bacteria physically associated with dinoflagellates and
more generally with phytoplankton. SUMMARY.

Book Reviews:
Robert C. Carpenter. Marine Biology: Function, Biodiversity, Ecology. J.
Phycol. 2002 38: 412-414.
Source: http://www.jphycol.org/

|Б?БЛ?ОГРАФ?Я / БИБЛИОГРАФИЯ / BIBLIOGRAPHY |

Флора и фауна водоемов и водотоков Баргузинского заповедника
(Аннотированные списки видов) // Флора и фауна заповедников СССР. Вып. 91.
М., 2000. 180 с. Тираж 250 экз.
В 91 выпуск "Флора и фауна заповедников СССР" вошла работа, посвященная
флоре водорослей Баргузинского заповедника:
А.Б. Бочка. Водоросли (под редакцией Г.Ф. Мазеповой). С.8-123.
Source: http://herba.msu.ru/

PROSEA: 15: CRYPTOGAMS: ALGAE
Edited by WF Prud'homme van Reine and GC Trono
PROSEA: Plant Resources of South-East Asia 15. 318 pages, figs, tabs.
To order on-line: http://www.nhbs.com/xbscripts/bkfsrch?search=107939
Price:GBP85 2001 NHBS Stock Code:#107939A hardback
Backhuys, Netherlands

CYANOBACTERIAL AND ALGAL METABOLISM AND ENVIRONMENTAL BIOTECHNOLOGY
Edited by T Fatma
272 pages.
To order on-line: http://www.nhbs.com/xbscripts/bkfsrch?search=123459
Price:GBP57.50 1999 NHBS Stock Code:#123459A hardback
Alpha Science
Source: http://www.nhbs.com/

НОВАЯ МОНОГРАФИЯ
Уважаемые коллеги! Вышла в свет коллективная монография, некоторые
сведения о которой я посылаю для информации в Альгологическом Вестнике. Она
вызвала большой интерес у специалистов, однако тираж ее ограничен (300
экз.).Стоимость одного экз. 20 грн. (+ почтовые расходы). Если это издание
вызовет интерес у специалистов, активно пользующихся Интренетом, мы готовы
поместить некоторые главы, или даже всю монографию на сайте Отдела проблем
качества водной среды ОФ ИНБЮМ (http://bioassay.narod.ru).
Анна Георгиевна Петросян
Ст.н.с. отдела Проблем качества водной среды
Одесского филиала Института биологии южных морей НАН Украины
Контактный адрес: biotest@ukr.net

Килийская часть дельты Дуная весной 2000 г.:

Состояние экосистем и последствия техногенных катастроф в бассейне

Одесса, 2001.
НАН Украины, Одесский филиал института биологии южных морей
128 с. Ил. 19, табл. 46, библиогр. 104 назв.

Редакционная коллегия выпуска:
Ответственный редактор: к.б.н. Б.Г.Александров.
Ю.П. Зайцев, академик НАН Украины,
А.К. Виноградов, д-р.биол.наук.,
Л.В. Воробьева, д-р.биол.наук.,
В.Н. Золотарев, д-р.биол.наук.,
В.А. Иваница, д-р.биол.наук., профессор,
Г.Г. Миничева, д-р.биол.наук.
Ответственный секретарь - А.Г. Петросян, канд. биол. наук

Аннотация

Монография подготовлена на базе материалов Государственной санитарно-
эпидемиологической станции Одесской области и Дунайской ГМО (г. Измаил),
данных комплексных экспедиционных исследований, проведенных силами
временного творческого коллектива под руководством к.х.н. Н.И. Рясинцевой
(ОФ ИнБЮМ НАН Украины) в период с 22 по 27 апреля 2000 г. и анализа
ретроспективных материалов. В работе дан анализ гидрологических,
гидрохимических и гидробиологических характеристик района нижнего течения
реки Дунай, Килийской дельты и авандельты. Показаны изменения загрязнения
цианидами и тяжелыми металлами в контролируемых створах, дана общая
санитарно-гигиеническая, экологическая и токсикологическая оценка качества
вод и донных отложений. На основании сравнения с аналогичными периодами
предыдущих лет показаны изменения качества среды и характеристик сообществ
фитопланктона, зоопланктона и макрозообентоса. Выявлены факторы
экологического риска прямо или косвенно связанные с техногенным
воздействием. Проведена предварительная оценка размеров платежей за сброс
загрязняющих веществ в поверхностные воды и ущерба рыбным запасам
вследствие аварийных сбросов предприятиями Румынии токсичных загрязняющих
веществ (цианиды, тяжелые металлы) в период с 31 января по 10 марта 2000 г.
Издание рассчитано на специалистов в области гидрологии, гидрохимии,
гидробиологии и экологии, а также охраны и рационального использования
водных и рыбных ресурсов.
Печатается по решению Ученого совета ОФ ИнБЮМ НАН Украины
ї Одесский филиал Института биологии южных морей НАН Украины
ISBN 966-02-2186-X


СОДЕРЖАНИЕ

ВВЕДЕНИЕ (Н.И. Рясинцева)
Глава 1. Район работ, программа и материалы исследований (Н.И. Рясинцева)
Глава 2. ГИДРОЛОГИЯ, ГИДРОХИМИЯ, ЗАГРЯЗНЕНИЕ
. Гидрологические и гидрохимические условия (В.В. Адобовский, О.Р. Крутько,
Н.И. Рясинцева, Т.И. Коновалова)
. Уровень загрязнения вод и донных отложений (П.Т. Савин, Н.И. Рясинцева,
Н.Ф. Подплетная, С.А. Доценко)
. Цианиды в воде и донных отложениях (Л.И. Засыпка, Л.А. Харина, Н.И.
Рясинцева, Ю.Е. Михалечко)
. Тяжелые металлы в воде и донных отложениях (Л.И. Засыпка, Л.А. Харина,
Н.И. Рясинцева, Л.Ю. Секундяк, Е.А. Павлова, Ю.Е. Чумак)
Глава 3. сообщества пелагиали и бентали
. Бактериопланктон и бактериобентос (Н.Г. Теплинская)
. Фитопланктон (Д.А. Нестерова, А.И. Иванов)
. Физиологическое состояние сообществ фитопланктона (И.А. Скрипник, Е.В.
Кирсанова)
. Зоопланктон (Л.Н. Полищук)
. Макрозообентос (И.А. Синегуб)
Глава 4. Интегральная оценка качества воды и донных отложений украинской
части р. Дунай в связи с последствиями техногенных катастроф (С.Е. Дятлов,
А.Г. Петросян, О.Ф. Ганган, М.С. Дятлова)
Глава 5.Оценка экономического ущерба от загрязнения
. Определение размера платежей за сброс загрязняющих веществ в воды
украинского участка р. Дунай (Ю.Е. Михалечко)
. Расчет ущерба, нанесенного рыбному хозяйству в результате загрязнения вод
украинского участка р. Дунай (С.Г. Бушуев, В.Е. Рыжко, Е.Г. Воля)
. Оценка экономического ущерба, нанесенного загрязнением популяциям
проходных осетровых бассейна р. Дунай (Ю.А. Лукаржевский)
. Оценка ущерба от потери нерестилищ в результате вынужденного прекращения
водоподачи в придунайские озера Украины (Г.Ю. Толоконников)
Заключение (Н.И. Рясинцева)
ЛИТЕРАТУРА
ПРИЛОЖЕНИЯ
Source: Анна Георгиевна Петросян: biotest@ukr.net


|?НТЕРНЕТ / ИНТЕРНЕТ / INTERNET |

Уважаемые коллеги!
Может быть вас заинтересует следующая информация, которую я нашла в Сети.
Может быть ее можно включить в Альговестник. Я подумала, а что если в АВ
делать небольшие обзоры интернет-страниц по альгологии? Там попадается
зачастую нужные и интересные вещи.

1. На сайте Американского фикологического общества есть раздел - The
Phycological Newsletter, где представлены некоторые pdf версии этого
журнала.
http://www.psaalgae.org/pubs/newsletter.html

2. На сайте Университета Нью Джерси имеется раздел "The Euglenoid Project"
http://bio.rutgers.edu/euglena/
Интересной частью этого проекта, помимо массы информации о эвгленовых
водорослях, являются определительные ключи для родов эвгленовых водорослей
и видов рода Euglena (свободный доступ, download). Ключи выполнены в виде
приложения программы Lucid. Свободно распространяемую версию этой программы
можно скачать на сайте
http://www.lucidcentral.com/

3. На сайте "The SilicaSecchiDisk" приводятся данные по исследованиям
золотистых и диатомовых водорослей. Имеются микрофотографии, база данных по
родам и видам (с возможностью поиска) и определительные ключи по Синуровым
водорослям и роду Mallomonas. Ключи также выполнены в виде приложения
программы Lucid.
http://silicasecchidisk.conncoll.edu/LucidKeys/keys.html
Хотелось бы обратить особое внимание на очень хорошее качество фотографий
водорослей.
Source: Анисимова Ольга Викторовна

|ВСЯЧЕСКИ ПРИВЕТСТВУЕСЯ |
|личное распространение! Разошлите этот выпуск по Сети друзьям, знакомым и |
|коллегам! Распечатайте его на принтере! Отнесите домой! Сделайте ксерокопии |
|и подарите бескомпьютерным друзьям! |

Для того, чтобы регулярно получать наши выпуски, Ви должны отправить
нам письмо электронной почтой по адресу levanets@mail.ru
следующегосодержания:
Фамилия, имя, отчество,
E-mail адрес,
Место работы, дожность, ученая степень и звание,
Научные интересы,
Служебный или контактный адрес, телефон/факс.
Указать в разделе для комментария: ALGOLOG-registration.
Если Вы имеете некоммерческую информацию, связанную с изучением,
использованием или охраной водорослей, хотите поделиться своими мыслями,
мнением, дать информацию о новых публикациях или изданиях, попросить совета
у коллег, если Вы планируете проведение конференции, семинара, школы или
знаете о таковых, обязательно нам напишите и об этом узнают все наши
читатели.
Планируется разбить материалы по следующим рубрикациям:
1) обзор новостей,
2) научные форумы,
3) научная периодика,
4) библиография,
5) дискуссии,
6) персоналии,
7) доска объявлений,
8) комментарии и рецензии,
9) экспедиции,
10) разное.
Если у Вас имеются замечания или пропозиции - пишите и мы их
внимательно рассмотрим. Вся информация будет расссылаться с соблюдением
авторских прав без права на коммерческое ее использование. Присылка на наш
адрес какой-либо информации будет рассматриваться как разрешение автора на
ее опубликование в АльгоВестнике.
Мы оставляем за собой право отбора полученной от наших читателей
информации без уведомления причин автору. Также мы не несем ответственности
за достоверность присланных нам материалов и не всегда согласны с мнением
автора. Вся переписка должна проводиться преимущественно по электронной
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переписка возможна и по почте).
Свои послания можно присылать на украинском, русском, белорусском,
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язык оригинала без перевода.
С уважением, наилучшими пожеланиями и надеждой на сотрудничество,
Анатолий Леванец
E-mail: levanets@mail.ru, levanets@ukr.net
Web-site: http://www.oasis.kiev.ua/levanets/

Адрес:
служебный:
Институт ботаники им. Н.Г. Холодного НАН Украины,
отдел альгологии,
ул. Терещенковская, 2,
г.Киев-4, ГСП, 01601, Украина
тел. (+044) 224-51-57

домашний:
ул. Доброхотова, д. 24, ком. 54,
г.Киев, 03142, Украина

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Мантурова Оксана: river@ibc.com.ua (for O. Manturova)
Ярмошенко Людмила: lyar@svitonline.com
Романенко Петро: petro@rom.alpline.kiev.ua
Демченко Едуард: botany@biocc.univ.kiev.ua (For E.N. Demchenko)
Михайлюк Тетяна: levanets@mail.ru
Селезнева Наталья: nsel@bsu.edu.ru
Горбул?н Олег: Oleg.S.Gorbulin@univer.kharkov.ua
Громакова Алла: gamulya@kharkov.ukrtel.net (for A.Gromakova)
В?нн?кова Ольга: gamulya@kharkov.ukrtel.net (for O.Vinnikova)
Садогурська Св?тлана ? Садогурський Серг?й: nbs1812@ukr.net
Масюк Над?я Прохор?вна.
Л?л?цька Галина Георг??вна.
Виноградова Оксана: oxanavinogradova@hotmail.com
Борисова Олена Володимир?вна.
Царенко Петро Михайлович: swasser@botan.kiev.ua (To P. Tsarenko)
Леонть?в Дмитро: protista@mail.ru
Петльований Олег.
Патова Елена Николаевна: patova@ib.komisc.ru
Кузяхметов Григорий Гильмиярович: KuzyakhmetovGG@bsu.bashedu.ru
Шарипова Марина Юрьевна: SharipovaMY@ic.bashedu.ru
Шкундина Фаина Борисовна: ShkundinaFB@bsu.bashedu.ru
Алейникова Мария Даниловна (редакция журнала "Альгология").
Теренько Галина Викторовна: galla@paco.net
Прибыловская Наталья Сергеевна: osozin@mail.grsu.grodno.by
Комулайнен Сергей Федорович: komsf@krc.karelia.ru
Дубовик Ирина Евгеньевна: DubovikIE@bsu.bashedu.ru
Анисимова Ольга Викторовна: flora_oa@mail.ru
Ляшенко Оксана Александровна: lyashenk@ibiw.yaroslavl.ru
Болдина Ольга Николаевна: olgab1999@mail.ru; olgab@freemail.ru
Хайбуллина Лилия Салаватовна: irekmdm@ufanet.ru
Ситникова Юлия Алексеевна: felina@farlep.net
Харитонов Вячеслав Гергиевич: sterba@online.magadan.su
Бухтиярова Людмила Николаевна: bukhtiyarova@ukrpost.net
Михайлова Татьяна Александровна: chaban@arh.ru
Иванова Анна Петровна: a.p.ivanova@ibpc.ysn.ru
Миничева Галина Григорьевна: minicheva@eurocom.od.ua
Дятлов Сергей Евгеньевич: biotest@ukr.net
Петросян Анна Георгиевна: Anna_Petrosyan@ukr.net
Солоненко Анатолий Николаевич: station@melitopol.net (for A.N. Solonenko)
Вознячук Ирина Петровна: ipv@biobel.bas-net.by
Витенайте Тересе: terese@botanika.lt
Шадрин Николай Васильевич: lib@ibss.iuf.net,
shadrn@fossil.ukrcom.sebastopol.ua
Козлова Дина Владимировна: kodv13@uic.nnov.ru, dinakozlova@rambler.ru
Леман Наталья, Челомбицкая Мария: maria@bio.donetsk.ua