Документ взят из кэша поисковой машины. Адрес оригинального документа : http://dualopt1.cmm.msu.ru/pub/Education/NanoMod/nucl_acid.pdf Дата изменения: Thu May 6 18:34:40 2010 Дата индексирования: Fri Feb 28 20:25:20 2014 Кодировка:

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() ­ , , .

2 1. ­ 2. ­


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. · ­ «» () . 1869 . · ­ . · ­ .


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HO
5 4 3

2

HO

O
2

OH
1

O HO P OH OH

OH
5 4 3


OH
1 2

O

O O P OH O
5 4 3

O
2

OH
1

OH


.
O
87 9

NH2 NH N NH N N

N NH

5 4

6

N
1 2

N NH N

N
3

NH2

P

u
3 2 4

r in

e

G

u
NH2 N NH

a

n

in
H3C

e
O NH NH

A

d

e
O

n

in
NH

e

N
5 6

NH
1

O

O

NH

O

P

y r im

id

i nC

y to

s i n Te h

ym

i n er a U

c


.
HO
5 4 3

O
2

OH
1
+ 2

HO

5 4 3

O

B



1 N- 2

O O P OH O
5 4 3

O O P OH


O
2

OH
1

O

5 4 3

O
2

B
1

OH

OH



.
NH2
4 3
O

NH
5 6

H O
5* 4* 3*

2

O

P OH

N O
1


O

1* N- 2*


HO


.
O
4 3
O

NH
5 6

H O
5* 4* 3*

2

O

P OH

N O
1


O

1* N- 2*

HO

OH




.
O


6

HN


OH P OH O

5 4

NH
1 2 3

O
5* 4* 3*

O

O

1* 2*



HO HO





O H3C

5'
O

OH P OH O O

T
N

NH

O

NH2 N

O O P OH O O

C
N O NH2 N O O P OH N O O P OH OH O O N O O N N

A
N O

NH

G
N NH2

3'


.
3'
N H2N O H3C N N OH O N N O O NH2 HN N O O P OH N O O P OH N O O P OH OH O O N O O N O O N O OH O P O CH3 O H2N NH2 HN N O O O P O O OH

A

5'
O

OH P OH O O

T
N

NH

G

N

C
N

T
O

N O O

OH P O O

A
N O

H2N N NH

C
O

N O O

OH P HO O

G
N NH2

5' 3'


.
N H2N O N NH
O O N HN NH NH

A N N
NH NH2

U

G
NH N

G
NH N NH2

O

O

NH

O

HN

U
O

NH

U
NH O


.


.
Watson Crick 1953 . . 1. . 2. . 3. .


.
.

1. ( ). . 2. - .


.




.
·Adenine ·Guanine ·Cytosine ·Thymine C6, N6, C5, N7, C5 C6, O6, C5, N7, C8 C6, C5, C4, N4 C6, C5, C4, O4, C5M
N H2 N O H3 C N NH O NH N
2

C2, N3, C4, N9 C2, N2, N3, C4, N9 O2, N1, C2 O2, C2, N1, C6
N NH HN N

A

G
H2 N

N

T
NH

NH

C
O NH O


.
- -


.
- -


.


.
-
2`

-
3`

3`

2`

C2'-endo

3`-endo



: ·- ·, 75% B A : -() > 75% , B A








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.
. 1. - . 2. . : , , .


.


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1. : ) . ) . ) ().

2. (HIV,).


.


.
(loop) (Pseudoknot)

(Internal loop) (stem) (junction)

(Bulge)


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GGCGACUGGUGAGUACGCC GGCGACUGGUGAGUACGCC


.
UU +5.9 / AA G C + ­2*2.9 / GC A + 3.3 / G C + ­1.8 / UA -0.9 / AU -1.8 / CG -2.1 / A U 3' A 5` 0 / A

5' G = -3.2 /


, .

. . .



1 2 3 2 1



()

- (kissing loops)

-

- ()

- ()














(B ) (A)
120 ENERGY = 18.0 5S_rRNA 1 U 0 2 119 1 2 G 1 3 118 2 3 C 2 4 117 3 ...................................... 116 G 115 117 4 116 117 G 116 118 3 117 118 C 117 119 2 118 119 A 118 120 1 119 120 U 119 0 0 120

(C)

(D)

(E )








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