|
E. A. Yakovleva, I. P. Andreeva, V. G. Grigorenko, A. P. Osipov
Immunochromatographic express-analysis of human heart fatty
acid binding protein for acute myocardial infarction
Abstract
As protein analyte the human heart
fatty acid binding protein (H-FABP) was chosen, which is a new and sensitive
marker for acute myocardial infarction. Rapid lateral flow immunoaassay based
on colloidal gold nanoparticles as a visual marker was developed for H-FABP
detection in serum. The detection limit of the method was 1,5 ng/ml, the
variation coefficient did not exceed 8%. With the developed test system was
analyzed a set of serum samples periodically withdrawn from the patients with
diagnosis of acute myocardial infarction (N = 10) over a period of 24 h after
admission to the hospital and the results were positive; in case of healthy
individuals (N = 25) test results were negative.
Copyright (C) Chemistry Dept., Moscow State University, 2002
|
|
