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Moscow University Chemistry Bulletin Vol. 52, No. 6, P. 432 (2011)
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E. A. Yakovleva, I. P. Andreeva, V. G. Grigorenko, A. P. Osipov

Immunochromatographic express-analysis of human heart fatty acid binding protein for acute myocardial infarction

Abstract

As protein analyte the human heart fatty acid binding protein (H-FABP) was chosen, which is a new and sensitive marker for acute myocardial infarction. Rapid lateral flow immunoaassay based on colloidal gold nanoparticles as a visual marker was developed for H-FABP detection in serum. The detection limit of the method was 1,5 ng/ml, the variation coefficient did not exceed 8%. With the developed test system was analyzed a set of serum samples periodically withdrawn from the patients with diagnosis of acute myocardial infarction (N = 10) over a period of 24 h after admission to the hospital and the results were positive; in case of healthy individuals (N = 25) test results were negative.
Moscow University Chemistry Bulletin.
2011, Vol. 52, No. 6, P. 432
   

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