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G. V. Presnova, M. Yu. Rubtzova, V. V. Shumyantzeva, T. V. Bulko, A. M. Yegorov
Comparative immobilization of antibodies on a surface of
modified screen-printed graphite electrodes
Abstract
The study of
immobilization of polyclonal antibodies on a surface of screen-printed graphite
electrodes (SPE) was carried out. The methods examined involved physical
adsorbtion and the immobilization in the films of DDAB and films of DDAB and
gold nanoparticles. The maximal electrochemical response was registered on the
SPE modified by films of DDAB and gold nanoparticles. We have found that such
films change markedly the surface morphology and the electrochemical signal is
more intensive and stable. In this case the concentration of the immobilized
antibodies increases, their activity remains. The detection limit for enzyme
label (horseradish peroxidase) was 0.02 ng/ml of probe.
Copyright (C) Chemistry Dept., Moscow State University, 2002
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