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Culturing of Mouse Mesenchymal Stem Cells on Poly-3-Hydroxybutyrate Scaffolds.

Article

Title:Culturing of Mouse Mesenchymal Stem Cells on Poly-3-Hydroxybutyrate Scaffolds.
Authors:Andreeva NV; Bonartsev AP; Zharkova II; Makhina TK; Myshkina VL; Kharitonova EP; Voinova VV; Bonartseva GA; Shaitan KV; Belyavskii AV
Publication:Bull Exp Biol Med. 2015 Aug;159(4):567-71. doi: 10.1007/s10517-015-3015-5. Epub 2015 Sep 21.
PubmedID26388561
Abstract
We studied the possibility of long-term culturing of mouse mesenchymal stem cells on a porous scaffold made of biocompatible polymer poly-3-hydroxybutyrate. The cells remained viable for at least 2 months and passed more than 65 population doublings in culture. Culturing on the scaffold did not change surface phenotype of cells. 3D poly-3-hydroxybutyrate scaffolds are appropriate substrate for long-term culturing of mesenchymal stem cells.