+-44-+-45-*AF2318
                   |    |                             
                   |    +-33-+-55-*APE1451
                   |         |                        
                   |         +-29-+-58-*MJ0213
                   |              |                   
                   |              +-37-+-9--*PH1876
                   |                   |              
                   |                   +-5--*PAB1135
                   |                                  
                   +-44-+-54-*MTH689
                        |                             
                        +-56-*VNG1287C
                                                    
                                                      
                  

Look at the tree. One of its branches is extracted.
It is supported by 29 positions - that's written on the scheme.
Let's consider some positions. E. g, in the position number 107 phenylalanine in sequences of the marked groups is placed opposite lysine in all other sequences (out of branch). It's an ideal case: alignment is divided into two clear groups, this division is the same with those, given by the branch.

More widespread is the case when positions consists of three or more amino acids. But on the one side of the branch (usually - inside the branch) one amino acid is placed. Such are positions number 5 (leucine opposite isoleucine, valine and methyonine), 30 (glutamic acid opposite arginine, glycine and serine), 51 (threonine opposite alanine, phenylalanine and serine), 55 (phenylalanine opposite leucine, histidine and tyrosine), 111 (phenylalanine opposite isoleucine, leucine and valine), 123 (valine opposite isoleucine, leucine and proline), 137 (lysine opposite glutamine, glutamic acid and valine), 139 (phenyalalnine opposite tyrosine and gap) and 155 (valine opposite alanine, isoleucine and leucine).
It's worth mentioning, that positions 30, 51, 111 and 137 are better that other ones - because two groups are presented by principially different acids (in position 5, for comparison, all aminos - isoleucine, leucine, methyonine and valine - are rather similar in their physico-chemical properties).

Positions of the third group (6, 7, 26, 38 - 42, 44, 60, 63, 68, 84, 91, 96, 106, 143, 154 and 158) are rather non-homogeneous. Though two classes of aminoacids (those which are present in the branch and these which present out of it) don't intersect, they are rather wide for clear distinquishing the branch. That's why it's no use paying attention on them.