Документ взят из кэша поисковой машины. Адрес оригинального документа : http://mccmb.belozersky.msu.ru/2013/abstracts/abstracts/90.pdf Дата изменения: Tue Mar 12 20:34:22 2013 Дата индексирования: Thu Feb 27 20:50:34 2014 Кодировка:

Mo lec ula r data co nfir m r ece nt fluc t uatio ns o f nor t her n bo rder o f d ice s nak e (Nat rix t essellat a) ra nge in Easte r n Europe

The d ic e s nake, Nat rix t essellat a is d istr ib ut ed fro m It a ly o ver t he Ba lka ns, Near East, to China. It pre fers aq uatic or mars hy hab ita ts, inc lud ing b rack is h wa ter. ). I n East er n Europe, t he nor t her n borde r o f co nt inuo us ra nge o f t he spec ies is bet wee n 49° N a nd 50 ° N la t it ud e. Ho we ver, t here a re so me iso lated pop ulatio ns more to t he nor t h, wit h t he mos t we ll k nown o f t he m in Ga lic hya Go ra Nature Rese r ve, the upp er Do n c urre nt, Lipe tsk province, Russ ia. In o ur stud y we used t he c yto c hro me o xidase s ub unit 1 barcod ing re gio n to e luc ida te the phylo ge netic re latio ns hip be twe e n pre vio us ly unco ve red Nat rix t essellat a populatio ns fro m Eas ter n Europe a nd t he Ba lka ns. We we re ab le to co mpare t he co ncorda nc e betwee n topo lo gy o f the p hylo ge netic tree based o n t he CO I seq ue nces a nd t he phylo ge netic tr ee based o n t he c ytoc hro me b fro m t he s t ud y o f Guick ing et a l. (2009). Samp les were take n fro m a nima ls ca ught in t he fie ld, whic h were s a fe ly re lea sed a fter t he proced ure. DNA wa s iso lated fro m t iss ue sa mp le s o f up to 5 mm o f d is ta l pa rt o f t he ta il. We a lso sa mp led t is s ues fro m sp ec ime ns o f muse um co llectio ns. We us ed in to ta l 15 spec ime ns o f N. t esse llat a. In o rder to obta in part ia l CO I ge ne s eq ue nce, we used t he d e ge nera te pr imer p a ir V1F and V1R p ub lis hed b y S mit h et a l (2008 ). Out o f t he 15 spec ime ns we ha ve fo und 8 hap lo t ypes p ro vided wit h t he fo llo wing access io n numbers : A (JN871603) : Ro1 ­ Ro5, Ser1, Ser2 ; B (JN871604) : Ser3; C

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(JN871605) : I ta; D (JN871606) : Gr3, E (JN871607) : Ukr1, Rus ; F (JN871608) : Ukr2 ; G (JN871609) : Gr1; H (JN871610) : Gr2 (F ig. 1 ).

The p hylo ge netic a na lys es o f t he N. t essellat a seq ue nc es ind icate 3 majo r c lad es. One gro up is fo r med b y t he Uk ra inia n a nd Russ ia n seq ue nce s a nd a not he r separ ate gro up b y the Gr1 a nd Gr2 seq ue nces, whic h are fro m t he ma inla nd o f Greece. It wa s s urp r is ing t ha t the third Greek sa mp le (Gr3 ), fro m t he is la nd o f Cre te, whic h is spatia lly c lo ser to t he Greek ma inla nd sa mp les (Gr1 a nd Gr2 ) c luster to ge t her wit h d is ta nt sa mp le s fro m Ro ma nia, I ta ly a nd Se rb ia. . Co nseq ue nt ly, t he Gr1 a nd Gr2 COI hap lot ype s fro m o ur stud y probab ly repre se nt t he re mna nts o f t he fir st co lo niza t io n a nd t he n s ur vived iso la ted s ince the early P lioce ne. O ur no rt he r nmos t a nd we ll iso lated pop ulatio n o f N. t essellat a fro m Ga lic hya Go ra ( Rus) see ms to be t he res ult fro m a ver y rec e nt a nd fast co lo nizatio n eve nt, as the t ime o f iso latio n was no t lo ng e no ugh to ga in a ny ge netic d ive r ge nce fro m the neares t Uk ra inia n sa mp le.

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