Research Group of  BIOCHEMISTRY of FIBRINOLYSIS Head of the Group: Dr. Roza B. Aisina

The major research directions of the group are as follows:

Enzymology and biochemistry of plasminogen activators (streptokinase, staphylokinase, urokinase and
pro-urokinase) inducing the fibrinolysis:
- isolation and purification of human plasma proteins;
- interaction kinetics of plasmin and plasminogen activators with substrates and inhibitors;
- kinetics and mechanism of human plasminogen activation by various plasminogen activators;
- inactivation and stabilization of plasmin and plasminogen activators;
- kinetics and mechanism of fibrinolysis induced by the plasminogen activators;
- influence of plasma components on the fibrinolysis kinetics;
- computer simulation of fibrinolysis.

Designing prolonged forms of thrombolytic agents:
- synthesis and purification of acylated by the active site derivatives of the plasminogen activators (urokinase,
   equimolar complexes of plasminogen-streptokinase and plasmin-staphylokinase) with different reactivation rates;
- chemical modification of molecules of the activators;
- investigation of fibrinolytic properties of the acylated and modified plasminogen activators and their side effects on
  plasma proteins levels.

Microencapsulation and microgranulation of biologically active substances:
- elaboration of methods for inclusion of the enzymes, proteins or drugs into semipermeable insoluble, biodegradable
  or intestine solvable microcapsules and microgranules;
- stabilization of enzyme and enzyme systems inside microcapsules and microgranules;
- designing microencapsulated and microgranulated preparations with time-controlled release.of active substance.

Elaboration of light-sensitive acyl-derivatives of proteases (plasmin, trypsin, chymotrypsin etc.) for biochemical
and biotechnological purposes.

List of publications  of the group includes more than 180 papers in national and international journals and 10 patents.

1. Aisina R.B. Effect of microencapsulated enzymes. In: "Microencapsulation of drugs", Ed. T.L.Whateley, V.1, Harwood Academic Pubs., Great Britain, 1992, P.215-232
2. Aisina R.B., Gaisarjan E.C., Snitko Ya.E., Varfolomeyev S.D. Inhibition by L-lysine of esterase, activator and fibrinolytic activities of plasminogen-streptokinase activator complex. Bioorg. Chemistry (Russia), 1993, V.20, N2, P.182-189.
3. Aisina R.B., Diomina N.B. Ovchinnikova T.N., Varfolomeyev S.D. Microencapsulation of somatotropic growth hormone. S.T.P. Pharma Sciences (France), 1994, V.4, N 1, P.1-6.
4. Aisina R.B.Snitko Ya.E., Jitkova Yu.V., Bashkov G.V.  New acyl-substituted derivative of streptokinase-plasmin activator complex with prolonged fibrinospecific thrombolytic action. In: Modern Enzymology Problems and Trends (B.I.,Kurganov, S.N.,Kochetkov and V.I. Tishkov Eds.). Nova Publishers, Inc., New York. 1994, P.741-745.
5. Sazonova I.Y., Tin U.F., Aisina R.B., Varfolomeyev S.D. Light-sensitive acyl-derivatives of human plasmin. Thromb. Haemost, 1995, V.73, N6, P.1132.
6. Jitkova Yu.V., Aisina R.B., Varfolomeyev S.D.  Kinetics of fibrinolysis by plasmin: inhibition by fibrin degradation products. Bioorg. Chemistry (Russia), 1996, V. 22, N12, P.911-915.
7. Moukhametova L.I., Aisina R.B. Varfolomeyev S.D.  Fibrinolysis by urokinase: effect of clot composition on the lysis kinetics.  Fibrinolysis, 1996, V.10, Suppl.3, P.121.
8. Aisina R.B., Moukhametova L.I., Varfolomeyev S.D. Acyl-derivatives of urokinase : kinetic properties in vitro. Thromb. Haemost., Suppl., P.192.
9. Aisina R.B., Moukhametova L.I., Sazonova I.Yu., Varfolomeyev S.D. Inactivation and stabilization of urokinase fibrinolytic activity in vitro. Bioorg. Chemistry (Russia),1998, V.24, N7, P.545-549.
10. Sazonova I.Y., Aisina R.B., Moukhametova L.I., Varfolomeyev S.D. Kinetic properties of equimolar complexes of plasminogen with staphylokinase and streptokinase in vitro. Biochemistry (Russia), 1999, V.64, P.83-93.

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