MAKING THE PROFILES . First of all you are expected to load a FITS file ("FILE"-"LOAD"). ... By pressing the middle button you copy the profile from the first profile window to the second one. ... An example: first integral can correspond to the profile of active region and second integral can correspond to the profile of quiet chromospheric region. ... The second profile (which is subtracted from the first one) you extract after clicking on "SUBTRACT"-"MARK QUIET PROFILE" and marking the region. ...