The isotopic shifts of helium spectral lines have been measured for four high velocity OB-stars and one standard star. The spectrograms were secured at 2.6m reflector of the Grimean observatory, dispersion beging 8 
/mm.
All five stars did not show the systematic isotopic shifts. But three lines of 53 Ari have shown shifts which are in agreement with theoretical ones, but other helium lines did not show such shifts. The possible explanation of such effec is discussed.