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Дата изменения: Mon Feb 13 17:55:34 2006
Дата индексирования: Mon Apr 11 05:32:32 2016
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Поисковые слова: m 19

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, , , . , , . , AI . : , (), () AI, . Stepwise discriminant analysis was performed in groups of middle-aged men with low and elevated high density lipoprotein (HDL) cholesterol (n=30 in each group). Mathematics models for cholesterol efflux calculation have been constructed. Apolipoprotein AI plasma concentrations appeared to be the most powerful parameter for prediction of the cholesterol acception activity as the first step of reverse cholesterol transport process. Key words: reverse cholesterol transport, high density lipoprotein cholesterol, apolipoprotein (apo) AI, regression models.

1. , (), . , () , , AI- () , . . . , , , : () () - [1-4]. - () , , , , , .

, - (ABCA1) [5, 6], ( , ) (), - (SR-B1) [6, 7]. - , [8]. , [9], [10] , , , , , , . , . 13

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2. - 40-59 , 5 ( 50 /), ( 40 /). , t- ( 0,05) ( 53,0±6,9 51,1±7,0, ). ( 96-1635) , . . [11], [12] [13] "Human" () "Airone-200" (). () [14] ( 400 /). I ("Behring", ), . II, AIV AI- - ("Sebia", ) [15-16]. AI: AII- AI AI- . "BoКhringer Mannheim" (). . - [3], [3H]- 14

Fu5AH. ( [17]). , , . , : - , [3]. () - - (130:30:1.5) ("Merk", ). , , . 12 0.5 MgCl2. , . , , , . . . , , -. ( , ) [18, 22]. F F- , [18, 22] =0,05. , [23] STATISTICA 5.5, SAS 6.0.

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. . , . . , . .

1. ( ± ) (/) B AI AII AIV AI AI:AII (%) (%) (%) a P < 0.05 I (n = 30) ( 50 /) 226 ± 6.7 101 ± 9.5 58 ± 1.6 9.9 ± 0.5 82.1 ± 2.7 148 ± 6.3 115 ± 2.9 133 ± 3.2 33 ± 1.3 62 ± 2.7 72 ± 1.7 111 ± 3.0
a a a a

II (n = 30) ( 40 /) 218 ± 6.1 159 ± 5.5 36 ± 0.8 11.6 ± 0.4 40.7 ± 1.2 150 ± 6.1 123 ± 3.1 101 ± 2.4 23.9 ± 0.93 23.1 ± 1.03 44 ± 1.3 58 ± 2.4 90 ± 3.1 9.7 ± 1.8

24.6 ± 1.66
a a a

8.8 ± 1.61 36.9 ± 1.22 7.0 ± 0.44 46.0 ± 2.7
a

31.5 ± 0.74 8.8 ± 0.3 49.0 ± 2.0

2. 0- ln ln ln ln ln ln ln F AI AII AIV = F0,95(1, 22)= 4,3 6.743 4.746 5.744 2.390 0.414 1.370 3.007 F ln AI (1- ) 0.917 5.257 0.828 0.176 0.103 1.349 ln AIV (2- ) 1.969 1.055 0.037 0.835 1.296

3. . 1 .

, , .. AI, AII, AIV, , (), (), .

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.

.

. 1. (1) 40 / () (2) 50 / (). (%). 95% .

, , , . . 2 3 , . . 2, F- 0- AI (ln AI), . . . 1- AI (AI) -

AIV (AIV). (F = F0,95(1,22) = 4.3). (%) . : Ln = 0,47 Ln AI + 0,16Ln AIV + 0,80 (1) (. 3) , Ln AI, - Ln AIV; : Ln C = 1,11 Ln AI 0,18 Ln AIV 1,26 (2) . 1 ,

3. ln ln ln ln ln ln ln F

AI AII AIV = F0,95(1, 22) = 4,3

0- 13.307 0.123 0.0139 3.535 2.942 0.203 1.865

F ln AI 1- 0.312 5.618 1.842 0.351 0.741 1.326

ln AIV 2- 1.303 0.784 0.010 0.357 0.337

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. . , . . , . .

() Ln C, (). AI AIV, (%) , , , . , , , , , , . ( ), . 1. . , , . , , (n=30) [22]. =0,05. . , . 1, . - . F- 0- AI ( ln AI) ( AI ( ln AI) , ), . . (%) . 1- AI ( )
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(I), (II). , I = 0, 21 AI + 8,49 (3)

c R=0,56 R2= 0,31 II = 0, 16 AI + 15,12 (4)

c R= 0,55 R2=0,30. (3)(4) AI, . , (%) AI : = 0, 18 AI + 13,13 (5)

R=0,69 R2=0,48 (. 2). 4. (1) (2), , , , AI AIV. , AIV (2) "", AIV (1) . [19], , [20, 21], . , , , 17

. 96-1635, - ( : . ., . ., . ., . ., . .; , . , : Castro G.; , , : Jauhiainen M.). . . . . .-. . . .
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. - 40 /; - 50 /. 95% .

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. . , . . , . . HDL-Cholesterol. Cardiovasc. Risk Factors 1997. V. 7. P. 1-5. Syvanne M., Castro G., Dengremont C. et al. Cholesterol efflux from Fu5AH hepatoma cells induced by plasma of subjects without coronary disease and noninsulin-dependent diabetes: importance of LpAI:AII particles and phospholipid transfer protein. Atherosclerosis 1996. V. 127. P. 245-253. Roeschlau P., Berndt E., Gruber W. Enzymatische bestimmung des gesant cholesterines im serum. Z Klin. Chem. Klin. Biochem. 1974. V. 12, P. 226-229. Bucolo G. Quantitative determination of serum triglycerides by use of enzymes. Clin. Chem. 1973. V. 19. P. 476-481. Assmann G., Schriewer H., Schmitz G., Hagele E. Quantification of high-density-lipoprotein cholesterol by precipitation with phosphotungstic acid/MgCl2. Clin. Chem. 1983. V. 29. P. 2025-2029. Friedwald W.T., Levy R.I., Fredrickson D.S. Estimation of the concentration of low-density lipoprotein in plasma without use of a preparative ultracentrifuge. Clin. Chem.1972. V. 18. P. 449-456. Parra H-J., Mezdour H., Ghalim N. et al. Differential electroimmunoassay of human Lp AI lipoprotein particles on ready-to-use plates. Clin. Chem. 1990. V. 36. P. 1431-1435. Bisgaier C.L., Sachdev O.P., Megna L., Glickman R. M. Distribution of apolipoprotein AIV in human plasma. J. Lipid Res. 1985. V. 26. P. 11-25. 17. .., .., .. . . . 2000. . 65 (. 11). . 1551-1557. 18. ., . . . . 1982. 19. Lagrost L., Dengremont C., Athias A. et al. Modulation of cholesterol efflux from Fu5AH hepatoma cells by the apolipoprotein content of high density lipoprotein particles. J. Biol. Chem. 1995. V. 270. P. 13004-13009. 20. Metelskaya V.A., Dehouck M.-P., Cecchelli R. et al. Lp AI, but not Lp AI:AII, promotes efflux of cholesterol from bovine aortic endothelial cells. Cardiovasc. Risk Factors. 1995. V. 5. P. 189-196. 21. Duverger N., Rader D., Ikewaki K. et al. Characterization of high density apolipoprotein particles AI and AI:AII isolated from humans with cholesteryl ester transfer protein deficiency. Eur. J. Biochem. 1995. V. 227. P. 123-129. 22. ., . . . 2. : , " ", 1987.

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